Modification and Characterization of Lactoferrin-Iron Free with Methylimidazolium N-ethylamine Ionic Liquid as Potential Drugs Anti SARS-CoV-2

Engineering Proceedings

article

Ahmed M. Senan¹  Senem Akkoc¹  Alariqi Reem⁴ 

[1] Department of Basic Pharmaceutical Sciences, Faculty of Pharmacy, Suleyman Demirel University;Department of Chemistry, Faculty of Science, Taiz University;Faculty of Engineering and Natural Sciences, Bahçeşehir University;Medical Laboratory Department, Faculty of Medical Sciences, Amran University;Yale School of Medicine, Yale University

关键词: ionic liquid;    infections;    lactoferrin;    N-glycans;    mass spectrophotometry;   

DOI  :  10.3390/ECP2023-14701

来源: mdpi

【 摘 要 】

Methylimidazolium N-ethylamine amine (MIE-NH2) is synthesized successfully with excellent yield in the high performances and green chemical process, using N-methylimidazole and tert-butyl N-(2-bromoethyl) carbamate as starting materials. Following the mechanism of reductive amination, using this ionic liquid as a suitable ligand for modification, N-glycans contain the carbonyl group of the oligosaccharides, and the activity of an ionic liquid is disclosed by mass spectrometric techniques. This work illustrates that methylimidazolium N-ethylamine as an ionic liquid linked to carbohydrates, including N-glycans in lactoferrin and its derivatives, for example, lactoferrin (BL iron free), have been selected as examples of glycoproteins. The detection of profiling linked to oligosaccharides and glycoproteins is performed using UPLC/ESI-QTOF and MALDI-TOF mass spectrometry. Moreover, the ionic synthesis with active amino-group and employed as a multifunctional modification of the oligosaccharide, and using the products as applicable small molecules therapeutics linked to GlcNAc and its derivatives. Modifying glycoproteins by adding IL-MIE-NH2 has improved ESI ionization efficiency and provided labeling results of N-glycans, even better than 2-AB derivatives. Relevantly, this ionic liquid is applicable as advancement and development in catalytic methods, N-glycosylation, and modification of small molecules as potential drugs against viral and microbial infections.

【 授权许可】

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