Rapid Simultaneous Determination of 11 Synthetic Cannabinoids in Urine by Liquid Chromatography-Triple Quadrupole Mass Spectrometry

Separations

article

Jianbing Wu¹  Fan Zhang²  Xing Ke¹  Wei Jia²  Xuzhi Wan²  Lange Zhang²  Yilei Fan¹  Jing Zhou¹ 

[1] Key Laboratory of Drug Prevention and Control Technology of Zhejiang Province, Zhejiang Police College;Key Laboratory of Agro-Products Postharvest Handling of Ministry of Agriculture and Rural Affairs, Zhejiang Key Laboratory for Agro-Food Processing, College of Biosystems Engineering and Food Science, Zhejiang University

关键词: synthetic cannabinoids;    LC-MS/MS;    simultaneous determination;    quantitative analysis;    urine;   

DOI  :  10.3390/separations10030203

学科分类：社会科学、人文和艺术（综合）

来源: mdpi

【 摘 要 】

Synthetic cannabinoids are a series of synthetic substances that mimic the effects of natural cannabinoids and produce a much stronger toxicity than natural cannabinoids, and they have become the most abused family of new psychoactive substances. A solid-phase extraction–liquid chromatography–triple quadrupole/linear ion trap mass spectrometry method has been developed to determine 11 synthetic cannabinoids in rat urine. Oasis HLB cartridge was selected to simultaneously extract synthetic cannabinoids for pretreatment. The effects of the loading solution and elution reagent volume on the recovery were investigated. The optimized acetonitrile proportion and elution reagent volume were determined by both high recovery and low solvent consumption. The results showed that the linear coefficients of determination of 11 types of synthetic cannabinoids ranged from 0.993 to 0.999, the limit of quantitation ranged from 0.01 to 0.1 ng/mL, and the spiked recoveries ranged from 69.90% to 118.39%. The research presented here provides a validated liquid chromatography tandem mass spectrometry method to accurately identify and quantitate synthetic cannabinoid metabolites in urine samples.

【 授权许可】

CC BY