Simultaneous Determination of Flavonoids in Different Parts of Citrus reticulata ‘Chachi’ Fruit by High Performance Liquid Chromatography—Photodiode Array Detection

Molecules

Yinshi Sun¹  Jianhua Wang¹  Shubo Gu¹  Zhengbo Liu¹  Yujie Zhang¹ 

[1] ¹State Key Laboratory of Crop Biology, College of Agronomy, Shandong Agricultural University, Taian, 271018, China ²Agricultural Science Research Institute of Shandong, Taian, 271000, China

关键词: high performance liquid chromatography–photodiode array detector (HPLC–PDA);    quantitative analysis;    flavonoids;    Citrus Reticulata ‘Chachi’ fruit;    simultaneous determination;   

DOI  :  10.3390/molecules15085378

来源: mdpi

【 摘 要 】

Flavonoids are important polyphenolic secondary metabolites in plant. Citrus reticulata ‘Chachi’ fruit are rich in flavonoids and are being used as functional antioxidant ingredients for the treatment of atherosclerosis and cancer, etc. A high performance liquid chromatography-photodiode array detection system was used to analyze five flavonoids, namely, naringin, hesperidin, didymin, tangeretin and nobiletin, in different parts of C. reticulata ‘Chachi’ fruit. The chromatographic analysis was performed on a C₁₈ column with a gradient elution of acetonitrile and water at a flow rate of 1.0 mL/min. Detection was carried out using a photodiode array detector at 280 nm. The calibration curves for the determination of all analytes showed good linearity over the investigated ranges (R² > 0.9995). Precision and reproducibility were evaluated by six replicated analyses, and the R.S.D. values were less than 0.9% and 2.7%. The recoveries were between 98.37 and 103.89%. This method is promising to improve the quality control of different parts of C. reticulata ‘Chachi’ fruit.

【 授权许可】

CC BY   
This is an open access article distributed under the Creative Commons Attribution License (CC BY) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.