Adsorption Properties and Preparative Separation of Flavonoids from Rhizoma Smilacis Glabrae Using Macroporous Resins

Separations

article

Xiangyi Su¹  Xuan Zhang¹  Chunhua Wang¹  Guige Hou¹  Hongjuan Li¹ 

[1] The Key Laboratory of Prescription Effect and Clinical Valuation of State Administration of Traditional Chinese Medicine of China, School of Pharmacy, Binzhou Medical University

关键词: adsorption characteristics;    flavonoids;    purification;    macroporous resin;    Rhizoma Smilacis Glabrae;   

DOI  :  10.3390/separations9120431

学科分类：社会科学、人文和艺术（综合）

来源: mdpi

【 摘 要 】

Astilbin (AST), isoastilbin (ISO), and engeletin (ENG) are the main flavonoids in Rhizoma Smilacis Glabrae (RSG) and have many biological activities. In this study, the adsorption kinetics of AST, ISO, and ENG on HPD-300 resin was investigated and their adsorption processes conformed to a pseudo-second-order kinetics equation. The fitting curves of the intraparticle diffusion model showed three linear stages and did not pass through the origin, meaning the adsorption process of the three flavonoids was controlled by boundary layer diffusion and intraparticle diffusion. Their adsorption isotherms were also constructed and could be well-fitted by the Langmuir equation. A low temperature was favorable for their adsorption. The relative adsorption capacity of ENG was significantly higher than those of the other two compounds, indicating that the substitution pattern on ring B has an important impact on the adsorption of flavonoids with resin. The separation process was optimized by dynamic adsorption/desorption experiments. After separation, the purities of AST, ISO, and ENG increased from 5.55%, 1.22%, and 0.45% to 27.46%, 6.14%, and 2.27%, respectively, and all the recoveries exceeded 75%. After that, the three compounds were further separated by preparative HPLC and silica gel chromatography. In the final product, the purities of AST, ISO, and ENG could reach above 98%.

【 授权许可】

CC BY