In Vitro and In Vivo Virulence Study of Listeria monocytogenes Isolated from the Andalusian Outbreak in 2019

Tropical Medicine and Infectious Disease

article

Andrea Vila Domínguez¹  Marta Carretero Ledesma¹  Carmen Infante Domínguez¹  José Miguel Cisneros¹  Jose A. Lepe¹  Younes Smani¹ 

[1] Institute of Biomedicine of Seville, IBiS, University Hospital Virgen del Rocio/CSIC/University of Seville;Clinical Unit of Infectious Diseases, Microbiology and Parasitology, University Hospital Virgen del Rocio/CSIC/University of Seville;CIBER de Enfermedades Infecciosas ,(CIBERINFEC), Instituto de Salud Carlos III;Department of Molecular Biology and Biochemical Engineering, Andalusian Center of Developmental Biology, CSIC, University of Pablo de Olavide

关键词: Listeria;    outbreak;    animal model;    virulence;    pathogenicity;   

DOI  :  10.3390/tropicalmed8010058

学科分类：社会科学、人文和艺术（综合）

来源: mdpi

【 摘 要 】

In 2019, the biggest listeriosis outbreak by Listeria monocytogenes (Lm) in the South of Spain was reported, resulting in the death of three patients from 207 confirmed cases. One strain, belonging to clonal complex 388 (Lm CC388), has been isolated. We aimed to determine the Lm CC388 virulence in comparison with other highly virulent clones such as Lm CC1 and Lm CC4, in vitro and in vivo. Four L. monocytogenes strains (Lm CC388, Lm CC1, Lm CC4 and ATCC 19115) were used. Attachment to human lung epithelial cells (A549 cells) by these strains was characterized by adherence and invasion assays. Their cytotoxicities to A549 cells were evaluated by determining the cells viability. Their hemolysis activity was determined also. A murine intravenous infection model using these was performed to determine the concentration of bacteria in tissues and blood. Lm CC388 interaction with A549 cells is non-significantly higher than that of ATCC 19115 and Lm CC1, and lower than that of Lm CC4. Lm CC388 cytotoxicity is higher than that of ATCC 19115 and Lm CC1, and lower than that of Lm CC4. Moreover, Lm CC388 hemolysis activity is lower than that of the Lm CC4 strain, and higher than that of Lm CC1. Finally, in the murine intravenous infection model by Lm CC388, higher bacterial loads in tissues and at similar levels of Lm CC4 were observed. Although a lower rate of mortality of patients during the listeriosis outbreak in Spain in 2019 has been reported, the Lm CC388 strain has shown a greater or similar pathogenicity level in vitro and in an animal model, like Lm CC1 and Lm CC4.

【 授权许可】

CC BY