Separations | |
Microbead-Beating Extraction of Polycyclic Aromatic Compounds from Seabird Plasma and Whole Blood | |
article | |
Ifeoluwa Idowu1  Zhe Xia1  Nipuni Vitharana1  Chris Marvin2  Philippe J. Thomas3  Gregg T. Tomy1  Vida Moradi1  Thor Halldorson1  | |
[1] Department of Chemistry, University of Manitoba;Water Science and Technology Directorate;Wildlife Landscape Science Directorate | |
关键词: polycyclic aromatic compounds; plasma; serum; seabirds; microbead-beating extraction; | |
DOI : 10.3390/separations10010048 | |
学科分类:社会科学、人文和艺术(综合) | |
来源: mdpi | |
【 摘 要 】
Seabirds are widely regarded as an invaluable bioindicator of environmental health. Matrices including eggs and feathers have been used as non-lethal means to assess contaminant burdens. We have developed a new approach for extraction of polycyclic aromatic compounds (PACs) from seabird plasma and serum based on automated microbead-beating homogenization and extraction. Commercially available bovine serum and plasma were purposely fortified with a suite of PACs separately at three dosing levels, placed inside a custom-made stainless-steel tube containing ceramic microbeads, and subjected to an extraction process using a Precellys tissue homogenizer. Tubes were shaken forcefully in three-dimensions, facilitating high mass-transfer of PACs from the matrix into the hexane extraction solvent. The accuracy of the method ranged from 55 to 120% and limits of detection and quantitation ranged from 0.1 to 8 and 0.2 to 27 pg/μL, respectively. The method exhibited good repeatability with both inter- and intra-day repeatability < 30%. The developed method represents an effective and efficient approach to extraction of PACs from important biological matrices.
【 授权许可】
CC BY
【 预 览 】
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RO202307010001978ZK.pdf | 1550KB | download |