Microbead-Beating Extraction of Polycyclic Aromatic Compounds from Seabird Plasma and Whole Blood

Separations

article

Ifeoluwa Idowu¹  Zhe Xia¹  Nipuni Vitharana¹  Chris Marvin²  Philippe J. Thomas³  Gregg T. Tomy¹  Vida Moradi¹  Thor Halldorson¹ 

[1] Department of Chemistry, University of Manitoba;Water Science and Technology Directorate;Wildlife Landscape Science Directorate

关键词: polycyclic aromatic compounds;    plasma;    serum;    seabirds;    microbead-beating extraction;   

DOI  :  10.3390/separations10010048

学科分类：社会科学、人文和艺术（综合）

来源: mdpi

【 摘 要 】

Seabirds are widely regarded as an invaluable bioindicator of environmental health. Matrices including eggs and feathers have been used as non-lethal means to assess contaminant burdens. We have developed a new approach for extraction of polycyclic aromatic compounds (PACs) from seabird plasma and serum based on automated microbead-beating homogenization and extraction. Commercially available bovine serum and plasma were purposely fortified with a suite of PACs separately at three dosing levels, placed inside a custom-made stainless-steel tube containing ceramic microbeads, and subjected to an extraction process using a Precellys tissue homogenizer. Tubes were shaken forcefully in three-dimensions, facilitating high mass-transfer of PACs from the matrix into the hexane extraction solvent. The accuracy of the method ranged from 55 to 120% and limits of detection and quantitation ranged from 0.1 to 8 and 0.2 to 27 pg/μL, respectively. The method exhibited good repeatability with both inter- and intra-day repeatability < 30%. The developed method represents an effective and efficient approach to extraction of PACs from important biological matrices.

【 授权许可】

CC BY