Stewartia pseudocamellia and Torilis japonica Extracts Inhibit RANKL-induced Osteoclastogenesis in RAW 264.7 Cells

Journal of the Korean Chemical Society

article

Anh-Thu Nguyen¹  Chun Soo Na²  Ki-Young Kim¹ 

[1]Department of Genetics and Biotechnology, Kyung Hee University

[2]Lifetree Biotech Co., Ltd

关键词: Stewartia pseudocamellia;    Torilis japonica;    Osteoporosis;    Osteoclasts;    RAW 264.7;   

DOI  :  10.5012/jkcs.2023.67.2.120

学科分类：化学（综合）

来源: Korean Chemical Society

【 摘 要 】

Osteoporosis is a disease that causes the weakening of bone by increasing porosity, which often results in fractures.Osteoporosis treatment measures include the use of Bisphosphonates and estrogen. However, these treatments cannot be usedin the long term as these treatments have adverse side effects. Therefore, there is a need to identify better and safer treatmentoptions. For this, 63 plant extracts were screened and among them, six extracts showed high anti-osteoclastic activity with lowcytotoxicity. Of these six extracts, three extracts, Cudrania tricuspidata (P371), Ulmus davidiana var. japonica (P401), andTorilis japonica (P411), showed more than 50 percent osteoclast inhibition. While the remaining, Stewartia pseudocamelliaextracts I and II (P370, P397) and Cuscuta chinensis (P418), showed moderate or between 40-50 percent osteoclast inhibition. Among all the extracts, Torilis japonica (P411) showed the highest inhibitory action against osteoclast development. Torilis japonica (P411) primary components include Kaempferol, Quercetin, and Luteolin, all proven to inhibit osteoclastogenesis.Stewartia pseudocamellia extracts I and II (P370 and P397) showed moderate or 44% osteoclast inhibition. Stewartia pseudocamellia extract II (P397) enhanced the growth of RAW 264.7 cells by 19%. Torilis japonica (P411) and Stewartia pseudocamellia extract II (P397) suppressed the expression of osteoclast-specific genes in RANKL-induced osteoclastogenesis in RAW246.7 cells. Torilis japonica (P411) extracts even increased osteoblast-specific RUNX2 gene expression. This results providethat six extracts could be used as a potential treatment option for osteoporosis disease with the extracts of Torilis japonica(P411) and Stewartia pseudocamellia (P397) as an ideal candidates. However, the combination of the extract with higherosteoclastic inhibition and less toxic effects with further analysis should be recommended.

【 授权许可】

Unknown