Application of high-performance liquid chromatographyto determine the concentration of α-tocopherol in theblood of cattle

Journal of Livestock Science

article

I.V. Artamonov¹  A.V. Platonov¹ 

[1] Vologda Research Center of the Russian Academy of Sciences;Vologda Institute of Law and Economics of the Federal Penitentiary Service of Russia

关键词: HPLC;    tocopherol;    cattle;    blood serum;    separation;    extraction;   

DOI  :  10.33259/JLivestSci.2022.261-266

学科分类：农业科学（综合）

来源: Journal of Livestock Science

【 摘 要 】

The purpose of the work was to develop within the laboratory method of analysis of α-tocopherol in theblood of animals corresponding to the laboratory equipment and the practical needs of the laboratory. The mainobjective of the study is to develop a method for the extraction and analysis of α-tocopherol in the blood serum ofcattle, which could be used in studies related to the assessment of animal health and their productivity. Since most ofthe techniques are somehow related to the use of mass spectrometry (due to the low concentration of α-tocopherol inthe blood), and the content of α-tocopherol is an important indicator in the system of evaluation criteria, an attemptwas made to adapt the existing methods of extraction and analysis to the conditions and instrument base of thelaboratory. In the process of work, the extraction of α-tocopherol in various variations was performed, the methodsalready described in scientific publications were tested. The result was an adapted extraction technique, as well aschromatographic analysis using fluorimetric detection, the separation conditions were selected. In particular, theselection of the composition of the rolling phase for the most efficient separation was carried out, which made itpossible to reduce the analysis time from 23 minutes to 18 minutes (relative to the previously used technique).

【 授权许可】

Unknown