Early detection of phosphorus deficiency stress in cucumber at the cellular level using chlorophyll fluorescence signals

Journal of Water and Land Development

article

Leszek Sieczko¹  Piotr Dąbrowski²  Katarzyna Kowalczyk³  Janina Gajc-Wolska³  Wojciech Borucki⁴  Monika Janaszek-Mańkowska⁵  Waldemar Kowalczyk⁶  Domenica Farci⁷  Hazem M. Kalaji⁷ 

[1] Warsaw University of Life Sciences – SGGW, Department of Biometry, Institute of Agriculture;Warsaw University of Life Sciences – SGGW, Department of Environmental Management, Institute of Environmental Engineering;Warsaw University of Life Sciences – SGGW, Department of Vegetable and Medicinal Plants, Institute of Horticultural Sciences;Warsaw University of Life Sciences – SGGW, Department of Botany, Institute of Biology;Warsaw University of Life – SGGW, Department of Fundamentals of Engineering and Power Engineering, Institute of Mechanical Engineering;The National Institute of Horticultural Research;Warsaw University of Life Sciences – SGGW, Department of Plant Physiology, Institute of Biology

关键词: abiotic stress;    chlorophyll fluorescence;    cucumber;    JIP test;    multi-dimensional statistical analyses;    photosynthesis;   

DOI  :  10.24425/jwld.2022.143734

学科分类：农业科学（综合）

来源: Instytut Technologiczno-Przyrodniczego / Institute of Technology and Life Sciences

【 摘 要 】

Abiotic stressors contribute to growth restriction and developmental disorders in plants. Early detection of the first signs of changes in plant functioning is very important. The objective of this study was to identify chlorophyll fluorescence parameters that change under phosphorus deficiency stress in cucumber. In this work, a trail to study the early changes caused by phosphorus deficiency in cucumber plants by analysing their photosynthetic performance is presented. Chlorophyll- a fluorescence (ChF) parameters were measured every 7 days for a period of 28 days. Measurements were made separately on young and old leaves and on cucumber fruit. Parameters that decreased during the stress were: p2G , PI abs , PI total , RE o /CS o , and TR o /CS o . P deficiency decreased total electron carriers per RC ( EC o /RC ), yields ( TR o /ABS ( F v /F m ), ET o /TR o , RE o /ET o , ET o /ABS and RE o /ABS ), fluxes ( RE o /RC and RE o /CS o ) and fractional reduction of PSI end electron acceptors, and damaged all photochemical and non-photochemical redox reactions. Principal component analysis revealed a group of ChF parameters that may indicate early phosphorus deficiency in cucumber plants. Our results are used in the discovery of sensitive bioindicators of phosphorus deficiency in cucumber plants. Most JIP test parameters are linked to mathematical equations, so we recommend using of advanced statistical tools, such as principal component analysis, which should be considered very useful for stress identification. It has also been shown to be more effective in multivariate methods compared to univariate statistical methods was demonstrated.

【 授权许可】

Unknown