The immunohistochemical detection of peroxiredoxin 1 and 2 in canine spontaneous vascular endothelial tumors

The Journal of Veterinary Medical Science

article

Narumi OTSUKA¹  Kairi ISHIMARU²  Mami MURAKAMI³  Minami GOTO²  Akihiro HIRATA¹  Hiroki SAKAI¹ 

[1] Laboratory of Veterinary Pathology, Joint Graduate School of Veterinary Sciences, Gifu University;Laboratory of Veterinary Pathology, Faculty of Applied Biological Sciences, Gifu University;Laboratory of Veterinary Clinical Oncology, Faculty of Applied Biological Sciences, Gifu University

关键词: dog;    hemangiosarcoma;    immunohistochemistry;    peroxiredoxin;   

DOI  :  10.1292/jvms.22-0102

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

Peroxiredoxin (PRDX) is an antioxidant enzyme family with six isoforms (PRDX1–6). The main function of PRDXs is to decrease cellular oxidative stress by reducing reactive oxygen species, such as hydrogen peroxide, to H2O. Recently, it has been reported that PRDXs are overexpressed in various malignant tumors in humans, and are involved in the development, proliferation, and metastasis of tumors. However, studies on the expression of PRDXs in tumors of animals are limited. Therefore, in the present study, we immunohistochemically investigated the expression of PRDX1 and 2 in spontaneous canine hemangiosarcoma (HSA) and hemangioma (HA), as well as in selected normal tissue and granulation tissue, including newly formed blood vessels. Although there were some exceptions, immunolocalization of PRDX1 and 2 in normal canine tissues was similar to those in humans, rats, or mice. In granulation tissue, angiogenic endothelial cells were strongly positive for PRDX1 and 2, whereas quiescent endothelial cells in mature vessels were negative. Both PRDX1 and 2 were significantly highly expressed in HSA compared to HA. There were no significant differences in the expression of PRDX1 and 2 among the subtypes and primary sites of HSA. These results suggest that PRDX1 and 2 may be involved in the angiogenic phenotypes of endothelial cells in granulation tissue as well as in the behavior in the malignant endothelial tumors.

【 授权许可】

Unknown