The profile of urinary lipid metabolites in healthy dogs

The Journal of Veterinary Medical Science

article

Taiki KIDA¹  Arisa YAMAZAKI¹  Koji KOBAYASHI¹  Tatsuro NAKAMURA¹  Takayuki NAKAGAWA²  Ryohei NISHIMURA²  Takahisa MURATA¹ 

[1] Department of Animal Radiology, Graduate School of Agricultural and Life Sciences, The University of Tokyo;Laboratory of Veterinary Surgery, Graduate School of Agricultural and Life Sciences, The University of Tokyo

关键词: dog;    lipid metabolite;    urine;   

DOI  :  10.1292/jvms.22-0020

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

Polyunsaturated fatty acids, including arachidonic acid (AA), docosahexaenoic acid (DHA), and eicosapentaenoic acid (EPA), are converted to hundreds of lipid mediators by cyclooxygenases (COX), lipoxygenases (LOX), and cytochrome P450 (CYP), or through non-enzymatic processes, and they reflect inflammatory states of the body. We comprehensively analyzed lipid metabolites in dog urine using a liquid chromatograph-mass spectrometry (LC-MS/MS) to describe their metabolic characteristics. We detected 31 AA-derived metabolites, four EPA-derived metabolites, and a DHA-derived metabolite in all urine samples. Among AA-derived metabolites, 15, 5, 3, and 8 were generated by COX, LOX, CYP, and non-enzymatic oxidation respectively. This study will be the first step to use profiles of urinary lipid metabolites for better understanding and diagnosis of canine diseases.

【 授权许可】

Unknown