Characterization of chicken IFI35 and its antiviral activity against Newcastle disease virus

The Journal of Veterinary Medical Science

article

Yan Qing JIA¹  Xiang Wei WANG²  Xi CHEN²  Xin Xin QIU¹  Xing Long WANG²  Zeng Qi YANG² 

[1] Department of Animal Engineering/Engineering Research Center of Animal Disease Prevention and Control, Universities of Shaanxi Province, Yangling Vocational & Technical College, Shaanxi Province;College of Veterinary Medicine, Northwest A&F University, Shaanxi Province

关键词: antivirus;    chicken;    interferon;    interferon-induced protein-35 kDa;    Newcastle disease virus;   

DOI  :  10.1292/jvms.21-0410

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

Interferon-induced protein-35 kDa (IFI35) was an antiviral protein induced by interferon (IFN)-γ, which plays an important role in the IFN-mediated antiviral signaling pathway. Here, we cloned and identified IFI35 in the chicken for the first time. Chicken IFI35 (chIFI35) contains an open reading frame (ORF) of 1,152 bp encoding a protein of 384 amino acids containing two conserved Nmi/IFI35 domain (NID) motifs. Tissue distribution analysis of chIFI35 in healthy and Newcastle disease (ND) virus-infected chickens indicated a positive correlation between chIFI35 mRNA transcription and ND viral loads in various tissues. The role of chIFI35 in regulation NDV replication were further assessed by up- or down-regulated chIFI35 expression in DF-1 cells transfected with plasmid harboring chIFI35, pCMV-3HA-chIFI35 or shRNA targeting chIFI35, pshRNA-chIFI35 plasmids. NDV replications in DF-1 cells were significantly reduced or slightly increased by over- or under-expression of the chIFI35 protein, respectively, indicating the role of chIFI35 in anti-NDV infection. Moreover, chIFI35 also involved in regulation of viral gene transcription and IFNs expression. The collected data were meaningful for research of chicken antiviral immunity and shed light on the pleiotropic antiviral effect of chIFI35 during NDV infection.

【 授权许可】

Unknown