Biological Effects of Chicken Type III Interferon on Expression of Interferon-Stimulated Genes in Chickens: Comparison with Type I and Type II Interferons

Journal of Veterinary Medical Science

Yasumitsu MASUDA²  Atsushi ASANO²  Toru SUGAI²  Yoshiaki YAMANO²  Akiko MATSUDA²  Tatsufumi USUI¹ 

[1] Laboratory of Veterinary Hygiene, School of Veterinary Medicine, Faculty of Agriculture, Tottori University, Tottori 680–8553, Japan;Laboratory of Veterinary Biochemistry, School of Veterinary Medicine, Faculty of Agriculture, Tottori University, Tottori 680–8553, Japan

关键词: CEF;    chicken;    interferon;    Mx;    OAS;   

DOI  :  10.1292/jvms.11-0517

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(32)Cited-By(7)Interferons (IFNs) are key mediators that activate host defense mechanisms against viruses. The recently identified mammalian Type III IFN has biological effects similar to type I IFN. However, the biological effects of type III IFN have not yet been characterized in birds. We compared the effects of chicken type III IFN (IFN-λ) with type I (IFN-β) and type II (IFN-γ) IFNs on IFN-stimulated genes (ISGs) using recombinant proteins expressed in Escherichia coli. Recombinant chicken IFN-λ inhibited influenza virus replication and induced the mRNA expression of the ISGs, Mx and OAS, in chicken embryonic fibroblasts (CEFs) in a dose-dependent manner. However, the effective dose of IFN-λ was higher than that of IFN-β and IFN-γ. Furthermore, the effect of IFN-λ on induction of Mx and OAS was lesser than that of IFN-β, but comparable to that of IFN-γ. These results indicate that chicken IFN-λ has the potential to induce ISGs and inhibit viral replication in chicken cells.

【 授权许可】

Unknown