Induced Toxicty and Bioaccumulation of Chromium (VI) in Cluster Bean: Oxidative Stress, Antioxidative Protection Strategy, Accumulation and Translocation of Certain Nutrient

Jordan Journal of Biological Sciences

article

Kamlesh Kumar Tiwari¹  Manoj Kumar Bidhar¹  Naveen Kumar Singh² 

[1]Sophisticated Instrumentation Centre for Applied Research and Testing ,(SICART),Sardar Patel Centre for Science and Technology, Vallabh Vidyanagar

[2]Department of Chemistry, Environmental Science Discipline Manipal University Jaipur- 303007

关键词: Chromium;    Phytotoxicity;    RWC;    Glutathione reductase;    Non protein thiol;   

DOI  :  10.54319/jjbs/160209

学科分类：生物科学（综合）

来源: Hashemite University, Deanship of Research and Higher Studies

【 摘 要 】

The present investigation was carried out to examine the noticeable phytotoxicity symptoms, growth behavior, metabolic changes, sulfur, phosphorus, iron, chromium translocation and accumulation in the cluster bean (Cyamopsis tetragonoloba L) plants under different concentration of Cr (VI) treatment. Initially plants were grown after sowing of seeds for 90 d with supplying essential nutrients solution. On the day 91 after plant growth, Cr treatment was given as potassium dichromate at 0.05 mM, 0.25 mM and 0.50 mM concentration along with a set of control (0.00 mM Cr). At 0.5 mM of Cr (VI), visual toxicity symptoms were noticed 8-9 days after exposure expressed as loss of turgor, old as well as central leaves became chlorotic, wilted and reductions in leaf dimension. Later phases of toxicity symptoms appear on newly young upper leaves followed by chlorosis and necrosis in patches at subsequent stages. After 15 days of Cr exposure, toxicity symptoms showed at lower levels (0.25 mM) with decreased leaf area, RWC, biomass, yield, concentration of chlorophyll, non-reducing sugar, protein nitrogen, total nitrogen, starch, protein, hill activity, glutathione reductase and non-protein thiol; however, the concentration of reducing sugar, total sugar, non-protein nitrogen, catalase activity, peroxidase, ribonuclease, acid phosphatase, proline and lipid peroxidation increased in the Cr treated plants. Excess levels of Cr concentration resulted in reduction of iron (Fe) accumulation in the leaves from 497to 176 µg/g-1dw along with sulfur (S) and phosphorus (P) translocation significantly at 0.50 mM Cr concentration. Maximum Cr accumulation recorded in the roots (197µg g-1 dw) and leaves (142 µg g-1 dw) and minimum in the shoots (69 µgg-1dw) at higher concentration of Cr (0.50 mM) and 30 days of treatment duration. The present study concludes that uptake and accumulation of Cr at higher concentration affect the plant growth, metabolic processes, translocation of essential nutrients, crop yield and may lead to health hazards.

【 授权许可】

CC BY