Physiological Response and Detection of Inh2 Gene in Dieng Red Potato (Solanum tuberosum L.) Affected by Frost

Jordan Journal of Biological Sciences

article

Wa Ode Kamillah¹  Hermin Pancasakti Kusumaningrum¹  Rejeki Siti Ferniah¹  Azalia Puspa Herida¹  Garinda Linggar Nasansia¹ 

[1] Department of Biology, Faculty of Science and Mathematics, Diponegoro University

关键词: Dieng;    electrolyte leakage;    chlorophyll;    Inh2;    frost;   

DOI  :  10.54319/jjbs/150113

学科分类：生物科学（综合）

来源: Hashemite University, Deanship of Research and Higher Studies

【 摘 要 】

In this study, the response of Dieng red potato plants to frost exposure was evaluated physiologically by testing electrolyte leakage and chlorophyll content, and molecularly by detection of Inh2 gene. The physiological response shows an increased electrolyte leakage rate and a significant decrease in chlorophyll content in plants exposed to frost compared to those grown in normal temperatures. Sequencing of Inh2 gene identifies four homologous Solanum tuberosum alleles. The difference in the order of Inh2 lies in the gap of 12 bp. Phylogenetic analysis shows that inh2 gene is in a cluster with INH2α*C allele and has evolved slowly from a common ancestor with S. lycopersicum. This is the first study on the physiological mechanisms of frost tolerance and the sequencing of Inh2 gene in Dieng red potatoes. Hopefully, this study will provide useful information for the breeding of low-sugar Dieng red potatoes.

【 授权许可】

CC BY