Isolation and Identification of Dibenzothiophene Desulfurizing Bacteria Occurring in Oil Contaminated Soils of Mechanical Workshops

Jordan Journal of Biological Sciences

article

Praveen Reddy P¹ 

[1] Department of Microbiology, Vivekananda Degree and PG College

关键词: Biodesulfurization;    Dibenzothiophene;    4S pathway;    dsz operon;    Streptomyces sp;    VUR PPR 101;    Streptomyces sp;    VUR PPR;   

DOI  :  10.54319/jjbs/140420

学科分类：生物科学（综合）

来源: Hashemite University, Deanship of Research and Higher Studies

【 摘 要 】

When fossil fuels are oxidized, a wide range of hazardous gases reach the atmosphere. Among these gases, sulfur oxides which are released because of oxidation of organosulfur compounds occurring in fuels become the sources of environmental pollution and acid rain. Hydrodesulfurization, a traditional practice employed for the removal of sulfur content from petroleum products during refining process is not eco-friendly and effective in removal of sulfur content, especially, recalcitrant organosulfur compounds like dibenzothiophene. Biodesulfurization, which employs microbes for the removal of sulfur from fossil fuels, is an eco-friendly method and a better alternative to hydrodesulfurization. The dibenzothiophene is treated as a model organosulfur compound for biodesulfurization studies. The present paper deals with the isolation of bacteria which exhibit dibenzothiophene biodesulfurization via 4S pathway from oil contaminated soils, detection of intermediates and end product of 4S pathway using Gas chromatography-Mass spectroscopy (GC-MS) in the DBT culture broths of isolates positive for gibb’s test, amplification of dsz operon genes which regulate 4S pathway in desulfurizing bacteria and identification of DBT desulfurizing bacteria by microscopic examination, biochemical tests and 16S rRNA gene anaylsis. Two DBT desulfurizing bacteria isolated were found positive for Gibb’s test and Dibenzothiophene sulfone (DBTO ) one of the intermediates and 2-Hydroxy biphenyl, the end product of 4S pathway were detected in DBT 2 ,containing culture broths of both the desulfurizing bacteria when subjected to GC-MS. In both, the bacteria dsz operon genes were detected. The two bacteria were identified as Streptomyces sp. VUR PPR 101 and Streptomyces sp. VUR PPR 102.

【 授权许可】

CC BY