Efficacy of Molecular Method for the Diagnosis of Community-Acquired Bacterial Meningitis in Children

Journal of Microbiology and Infectious Diseases

article

Shahid Raza¹  Bimal Kumar Das¹  Rama Chaudhry¹  Vinay Goyal²  Rakesh Lodha³  Seema Sood¹  Hitender Gautam¹  Arti Kapil¹ 

[1]Department of Microbiology, All India Institute of Medical Sciences. New Delhi

[2]Department of Neurology, All India Institute of Medical Sciences. New Delhi

[3]Department of Pediatrics, All India Institute of Medical Sciences. New Delhi

关键词: Streptococcus pneumoniae;    Community-acquired bacterial meningitis;    CSF;    Real-Time PCR;   

DOI  :  10.5799/jmid.1130082

学科分类：生物科学（综合）

来源: Fatih University, Faculty of Medicine

【 摘 要 】

Objectives: Objectives: Community-acquired bacterial meningitis (CABM) is a life-threatening condition and remains a public health concern despite various efforts to prevent it. This study aimed to detect the bacteria causing CABM in children by Real-Time PCR. Methods: In total, 178 Cerebrospinal fluid (CSF) samples from suspected meningitis cases were collected and subjected to cell count, biochemical, microbiological, and molecular analysis. Bacteria grown on blood and chocolate agar were identified by matrix-assisted laser desorption ionizationtime of flight mass spectrometry (MALDI-TOF MS). DNA from CSF was extracted and used to detect bacteria by Real-Time PCR using TaqMan Probe. Results: Fifty (28.1%) patients were diagnosed with confirmed meningitis. Of them, 46 (25.8%) were Real-Time PCR, and four (2.3%) were culture and Real-Time PCR positive. Out of 50 bacteria detected, S. pneumoniae (n=35, 19.7%) was the leading causative bacteria and was followed by H. influenzae (seven, 3.9%), E. coli (five, 2.8%), S. agalactiae (two, 1.1%), and N. meningitidis (one, 0.6%). Most of the S. pneumoniae (18 isolates, 51.4%) were isolated from 3-24 months of children, and in neonates, E. coli was the predominant bacteria. When CSF culture was the gold standard for diagnosis, the sensitivity and specificity of Real-Time PCR for S. pneumoniae were 100% (95%CI: 15.8-100%) and 81.3% (95%CI: 74.7-86.7%), respectively.

【 授权许可】

CC BY-NC