Peculiarities of the genetically modified organisms’ detection in seed batches of rape (Brassica napus L.)

Plant Varieties Studying and Protection

Л. В. Король¹  О. В. Піскова¹  С. О. Гончарова¹  Л. М. Кожемякіна¹  А. В. Костенко¹  І. І. Коровко¹ 

[1] Ukrainian Institute for Plant Variety Examination;

关键词: rape;    GMO;    screening sequences;    Real-Time PCR;    cauliflower mosaic virus (CaMV);   

DOI  :  10.21498/2518-1017.1(22).2014.56608

来源: DOAJ

【 摘 要 】

This paper presents the results of molecular studies on testing and evaluation of multiplex test systems for the genetically modified organisms’ (GMOs) identification by PCR in real time. For the detection of genetic modifications in plants of the cabbage family (Brassicaceae) screening of major regulatory sequences is insufficient, as in genetic engineering manipulations 35S promoter – of DNA-containing cauliflower mosaic virus (CaMV) is used, which presence in the test material can lead to false positive results. In this work the feasibility of testing for the presence of viral CaMV’s DNA and analysis of screened gene sequences of interest for the usage during the analysis of rapeseed (Brassica napus L.) samples. It was established that the analysis of the presence/absence of a positive result for the CaMV 35S promoter is not giving complete answers to the absence of GMO in the sample. The appropriateness of GMO’s screening in rape seeds with NOS terminator and genes of interest.

【 授权许可】

Unknown