Sensitive Determination of Venlafaxine in Urine Samples by Using HPLC-DAD System After Fabric Phase Sorptive Extraction

Cumhuriyet Science Journal

article

Beyzanur ÖZDEMİR¹  Halil İbrahim ULUSOY¹  Ümmügülsüm MORGÜL²  Marcello LOCATELLݳ  Abuzar KABİR⁴ 

[1] SİVAS CUMHURİYET ÜNİVERSİTESİ;Sivas Cumhuriyet Üniversitesi;Department of Pharmacy, University of Chieti–Pescara “G. d’Annunzio”;Department of Chemistry and Biochemistry, International Forensic Research Institute, Florida International University

关键词: HPLC;    Fabric phase sorptive extraction;    Venlafaxine;    Analytical method development;   

DOI  :  10.17776/csj.1182848

学科分类：工程和技术（综合）

来源: Cumhuriyet University

【 摘 要 】

This study is focused on developing a sensitive analytical method for Venlafaxine molecule which is antidepressant drug belonging to the serotonin-noradrenaline reuptake inhibitors (SNRI) group. With this study, a separation and preconcentration method based on fabric phase sorptive extraction(FPSE) method was developed for low levels of Venlafaxine in urine samples. Experimental variables such as pH, ionic strength, desorption solvent, and other parameters were studied and optimized step by step. The linearity of method under optimized conditions is in the range of 15.00-750.00 ng mL-1 while limit of detection is 4.28 ng mL-1. The relative standard deviation (RSD %) obtained from model solutions containing 300 ng mL-1 of Venlafaxine was lower than 3.1 % and pre-concentration factor was calculated as 62.50 for target molecule. In order to test accuracy of the method, recovery tests were carried out by means of spiked urine samples. As a result of recovery tests, quantative values were obtained in the range of 97.5-104.2 % successively.

【 授权许可】

CC BY-NC-ND