Wnt3a-induced ST2 decellularized matrix ornamented PCL scaffold for bone tissue engineering

Biocell

article

XIAOFANG WANG¹  XIAOLIN TU¹  YUFEI MA¹  JIE CHEN¹  YANG SONG³  GUANGLIANG LIU¹ 

[1] Laboratory of Skeletal Development and Regeneration, The Institute of Life Science, Chongqing Medical University;Department of Anatomy and Cell Biology, Indiana University School of Medicine;Bioengineering Collage, Chongqing University

关键词: Wnt3a;    Decellularized matrix;    Bone marrow stromal cells;    Osteogenic differentiation;    Bone tissue engineering;   

DOI  :  10.32604/biocell.2022.020069

学科分类：仪器

来源: Biocell

【 摘 要 】

The limited bioactivity of scaffold materials is an important factor that restricts the development of bone tissue engineering. Wnt3a activates the classic Wnt/β-catenin signaling pathway which effects bone growth and development by the accumulation of β-catenin in the nucleus. In this study, we fabricated 3D printed PCL scaffold with Wnt3a-induced murine bone marrow-derived stromal cell line ST2 decellularized matrix (Wnt3a-ST2-dCM-PCL) and ST2 decellularized matrix (ST2-dCM-PCL) by freeze-thaw cycle and DNase decellularization treatment which efficiently decellularized >90% DNA while preserved most protein. Compared to ST2-dCM-PCL, Wnt3a-ST2-dCM-PCL significantly enhanced newly-seeded ST2 proliferation, osteogenic differentiation and upregulated osteogenic marker genes alkaline phosphatase (Alp), Runx2, type I collagen (Col 1) and osteocalcin (Ocn) mRNA expression. After 14 days of osteogenic induction, Wnt3a-ST2-dCM-PCL promoted ST2 mineralization. These results demonstrated that Wnt3a-induced ST2 decellularized matrix improve scaffold materials’ osteoinductivity and osteoconductivity.

【 授权许可】

CC BY