Intraoperative evaluation of metastases in sentinel nodes through one-step nucleic acid amplification: Initial experience in a single institution

Bratislava Medical Journal

article

Kamil POHLODEK¹  Michaela FERIANCOVA¹  Klara KOTVANOVA²  Iveta MECIAROVA² 

[1] Second Department of Gynaecology and Obstetrics, Comenius University of Bratislava, Faculty of Medicine;Unilabs Slovakia, Ltd., Center for Diagnostic Pathology

关键词: breast cancer;    metastases;    surgery;    sentinel nodes;    OSNA;   

DOI  :  10.4149/BLL_2023_026

学科分类：医学（综合）

来源: AEPress, s.r.o.

【 摘 要 】

The objective of this study was to gain our initial experience in one-step nucleic acid amplification (OSNA) for detecting sentinel lymph node (SLN) metastasis as compared to standard pathological staging in patients with breast cancer. Fifteen patients with preoperatively confirmed early breast cancer eligible for breast‑saving therapy and sentinel lymph node biopsy (SLNB) were enrolled in the study. Lymphatic mapping and SLNs detection were performed through the magnetic method. Excised SLNs were intraoperatively examined through OSNA and frozen‑section methods. All lymph nodes were postoperatively examined through histopathology and immunohistochemistry. The results of latter methods were correlated. Our initial experience proved OSNA to be a sensitive and efficient alternative to intraoperative assessment of metastases in SLN in breast cancer patients. Moreover, the information obtained by the OSNA method provides the surgeon with the possibility of assessing a more accurate prognosis during the initial surgery (Tab. 3, Fig. 4, Ref. 36).

【 授权许可】

CC BY