ESCRT-III controls nuclear envelope reformation

Article

关键词: AURORA-B;    ENDOPLASMIC-RETICULUM;    HELA-CELLS;    CYTOKINESIS;    PROTEIN;    ABSCISSION;    MACHINERY;    CHROMATIN;    DYNAMICS;    KINASE;   

DOI  :  10.1038/nature14503

来源: SCIE

【 摘 要 】

During telophase, the nuclear envelope (NE) reforms around daughter nuclei to ensure proper segregation of nuclear and cytoplasmic contents1-4. NE reformation requires the coating of chromatin by membrane derived from the endoplasmic reticulum, and a subsequent annular fusion step to ensure that the formed envelope is sealed(1,2,4,5). How annular fusion is accomplished is unknown, but it is thought to involve the p97 AAA-ATPase complex and bears a topological equivalence to the membrane fusion event that occurs during the abscission phase of cytokinesis(1,6). Herewe show that the endosomal sorting complex required for transport-III (ESCRT-III) machinery localizes to sites of annular fusion in the forming NE in human cells, and is necessary for proper post-mitotic nucleocytoplasmic compartmentalization. The ESCRT-III component charged multivesicular body protein 2A (CHMP2A) is directed to the forming NE through binding to CHMP4B, and provides an activity essential for NE reformation. Localization also requires the p97 complex member ubiquitin fusion and degradation 1 (UFD1). Our results describe a novel role for the ESCRT machinery in cell division and demonstrate a conservation of themachineries involved in topologically equivalent mitotic membrane remodelling events.

【 授权许可】

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