NEUROTROPHIN-6 IS A NEW MEMBER OF THE NERVE GROWTH-FACTOR FAMILY

Article

关键词: EMBRYONIC-DEVELOPMENT;    SENSORY NEURONS;    CELL-SURFACE;    IDENTIFICATION;    SURVIVAL;    CLEAVAGE;    PROTEINS;    RECEPTOR;    BINDING;    BIOLOGY;   

DOI  :  10.1038/372266a0

来源: SCIE

【 摘 要 】

DURING vertebrate development, many neurons depend for survival and differentiation on their target cells(1-3). The best documented mediator of such a retrograde trophic action is the neurotrophin nerve growth factor (NGF)(1). NGF and the other known members of the neurotrophin family, brain-derived neurotrophic factor (BDNF), neurotrophin-3 (NT-3) and neurotrophin-4/5 (NT-4/5) are conserved as distinct genes over large evolutionary distances(4-6). Here we report the cloning of neurotrophin-6 (NT-6), a new member of this family from the teleost fish Xiphophorus. NT-6 distinguishes itself from the other known neurotrophins in that it is not found as a soluble protein in the medium of producing cells. The addition of heparin (but not chondroitin) effects the release of NT-6 from cell surface and extracellular matrix molecules. Recombinant purified NT-6 has a spectrum of actions similar to NGF on chick sympathetic and sensory neurons, albeit with a lower potency. NT-6 is expressed in the embryonic valvulla cerebelli; expression persists in some adult tissues. The interaction of NT-6 with heparin-binding molecules may modulate its action in the nervous system.

【 授权许可】

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