Signal integration by Ca2+ regulates intestinal stem-cell activity

Article

关键词: TISSUE HOMEOSTASIS;    NEURAL ACTIVITY;    LIFE-SPAN;    DROSOPHILA;    GLUTAMATE;    PROLIFERATION;    CALCINEURIN;    CA2+-ATPASE;    METABOLISM;    INSIGHTS;   

DOI  :  10.1038/nature16170

来源: SCIE

【 摘 要 】

Somatic stem cells maintain tissue homeostasis by dynamically adjusting proliferation and differentiation in response to stress and metabolic cues. Here we identify Ca2+ signalling as a central regulator of intestinal stem cell (ISC) activity in Drosophila. We show that dietary l-glutamate stimulates ISC division and gut growth. The metabotropic glutamate receptor (mGluR) is required in ISCs for this response, and for an associated modulation of cytosolic Ca2+ oscillations that results in sustained high cytosolic Ca2+ concentrations. High cytosolic Ca2+ concentrations induce ISC proliferation by regulating Calcineurin and CREB-regulated transcriptional co-activator (Crtc). In response to a wide range of dietary and stress stimuli, ISCs reversibly transition between Ca2+ oscillation states that represent poised or activated modes of proliferation, respectively. We propose that the dynamic regulation of intracellular Ca2+ levels allows effective integration of diverse mitogenic signals in ISCs to adapt their proliferative activity to the needs of the tissue.

【 授权许可】

Free