The developmental transcriptome of Drosophila melanogaster

Article

关键词: GENE-EXPRESSION;    FUNCTIONAL ELEMENTS;    BITHORAX COMPLEX;    ABDOMINAL-B;    IDENTIFICATION;    GENOMES;    TARGETS;   

DOI  :  10.1038/nature09715

来源: SCIE

【 摘 要 】

Drosophila melanogaster is one of the most well studied genetic model organisms; nonetheless, its genome still contains unannotated coding and non-coding genes, transcripts, exons and RNA editing sites. Full discovery and annotation are pre-requisites for understanding how the regulation of transcription, splicing and RNA editing directs the development of this complex organism. Here we used RNA-Seq, tiling microarrays and cDNA sequencing to explore the transcriptome in 30 distinct developmental stages. We identified 111,195 new elements, including thousands of genes, coding and non-coding transcripts, exons, splicing and editing events, and inferred protein isoforms that previously eluded discovery using established experimental, prediction and conservation-based approaches. These data substantially expand the number of known transcribed elements in the Drosophila genome and provide a high-resolution view of transcriptome dynamics throughout development.

【 授权许可】

Free