MLXIPL promotes the migration, invasion, and glycolysis of hepatocellular carcinoma cells by phosphorylation of mTOR

BMC Cancer

Research

Chang Tian¹  Yuanyuan Jia²  Pu Yan³  Yu Cai³  Xiaowei Chang³ 

[1] Department of Clinical Laboratory, The First Affiliated Hospital of Xi’an Medical University, Xi’an, Shaanxi, China;Department of Faculty Development and Teaching Evaluation Office, The First Affiliated Hospital of Xi’an Medical University, Xi’an, Shaanxi, China;Department of General Surgery, The First Affiliated Hospital of Xi’an Medical University, No. 48, Fenghao West Road, Lianhu District, 710077, Xi’an, Shaanxi, China;

关键词: Hepatocellular carcinoma;    MLXIPL;    mTOR;    Glycolysis;    Invasion;    Migration;   

DOI  :  10.1186/s12885-023-10652-5

 received in 2022-11-22, accepted in 2023-02-15,  发布年份 2023

来源: Springer

【 摘 要 】

BackgroundHepatocellular carcinoma (HCC) is associated with a high occurrence, mortality, and poor prognosis. MLX interacting protein like (MLXIPL) is an important regulator of glucolipid metabolism and is involved in tumor progression. We aimed to clarify the role of MLXIPL in HCC and its underlying mechanisms.MethodsThe level of MLXIPL was predicted using bioinformatic analysis and verified using quantitative real-time PCR (qPCR), immunohistochemical analysis, and western blot. We assessed the effects of MLXIPL on biological behaviors using the cell counting kit-8, colony formation, and Transwell assay. Glycolysis was evaluated using the Seahorse method. The interaction between MLXIPL and mechanistic target of rapamycin kinase (mTOR) was confirmed using RNA immunoprecipitation and co-immunoprecipitation. mTOR expression was detected in HCC cells using qPCR, immunofluorescence analysis, and western blot.ResultsThe results showed that MLXIPL levels were elevated in both HCC tissues and HCC cell lines. Knockdown of MLXIPL impeded HCC cell growth, invasion, migration, and glycolysis. Moreover, MLXIPL combined with mTOR to induce phosphorylation of mTOR. Activated mTOR abrogated the effects on cellular processes induced by MLXIPL.ConclusionMLXIPL promoted the malignant progression of HCC by activating phosphorylation of mTOR, suggesting an important role of the combination of MLXIPL and mTOR in HCC.

【 授权许可】

CC BY   
© The Author(s) 2023

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