Forkhead box P3 gene polymorphisms predispose to type 2 diabetes and diabetic nephropathy in the Han Chinese populations: a genetic-association and gender-based evaluation study

Hereditas

Research

Zejing Liu¹  Shangdi Zhang¹  Yinfeng Yang²  Xiaorong Wang²  Xinyue Liu³  Xue Wu⁴ 

[1] Department of Clinical Laboratory Center, Lanzhou University Second Hospital, 730030, Lanzhou, Gansu, China;Department of Pharmacogenomics Laboratory Center, Lanzhou University Second Hospital, 730030, Lanzhou, Gansu, China;Department of Pharmacogenomics Laboratory Center, Lanzhou University Second Hospital, 730030, Lanzhou, Gansu, China;Department of Clinical Laboratory Center, Lanzhou University Second Hospital, 730030, Lanzhou, Gansu, China;Lanzhou University Second Hospital, 730030, Lanzhou, Gansu, China;

关键词: FOXP3;    T regulator cells;    Gender-based evaluation;    Single nucleotide polymorphism;    Type 2 diabetes mellitus;    Diabetic nephropathy;   

DOI  :  10.1186/s41065-023-00264-1

 received in 2022-09-05, accepted in 2023-01-13,  发布年份 2023

来源: Springer

【 摘 要 】

BackgroundFunctional mutations or polymorphisms affecting forkhead box P3 (FOXP3) can lead to their abnormal FOXP3 gene expression and/or defective Treg cells generation, thus resulting in autoimmune disease and inflammatory disorders. FOXP3 also plays a key role in Type 2 diabetes mellitus (T2DM) and its complications, because the disease usually involves chronic low-grade inflammatory disorders and is associated with long-term immune system imbalance. This study aimed to investigate the association between FOXP3 polymorphisms and the susceptibility to T2DM and type 2 diabetes nephropathy (T2DN) within the Han Chinese populations.MethodsPolymorphisms in rs3761548C/A and rs2294021C/T were examined in 400 patients (which include an equal number of T2DM and T2DN groups) and 200 healthy controls using PCR-HRM and sequence analysis.ResultsThe genotype and allelic frequencies of the two single nucleotide polymorphisms (SNPs) were significantly different in T2DM and the progression of diabetes developing to T2DN. The further gender-based evaluation showed that in female subjects, rs3761548C/A was associated with an approximately 3-fold higher threat for T2DM and 4.5-fold for T2DN, while there was no noticeable association with rs2294021C/T; in males, the promoter polymorphism showed an increased predisposition of 5.4-fold and 3.4-fold predisposition to T2DM and T2DN, respectively, while rs2294021 polymorphism could impart a nearly 2-fold risk of developing T2DN. An additional analysis of combined genotypes (rs3761548 C/A-rs2294021C/T) revealed that CC-CC and CC-CT can be considered protective combinations in the predisposition of males with diabetes towards T2DN, while AA-CC and AA-TT have the opposite effect.ConclusionsThis study demonstrated the possible involvement of individual and combined genetic associations of rs3761548C/A and rs2294021C/T polymorphisms with the susceptibility to diabetes and diabetic nephropathy in the Han Chinese population, as well as gender bias.

【 授权许可】

CC BY   
© The Author(s) 2023

【 图 表 】

Fig. 4