The Changes of Angiogenesis and Immune Cell Infiltration in the Intra- and Peri-Tumoral Melanoma Microenvironment

International Journal of Molecular Sciences

Vladimir Zidlik²  Svetlana Brychtova¹  Magdalena Uvirova²  Dusan Ziak²  Jana Dvorackova² 

[1]Institute of Clinical and Molecular Pathology, Faculty of Medicine and Dentistry, Palacky University Olomouc, Hnevotinska 3, Olomouc 77515, Czech Republic

[2]CGB Laboratory, a.s., Laboratory of Molecular Genetics and Pathology, AGEL Research and Training Institute—Ostrava-Vitkovice Branch, Korenskeho 10, Ostrava 71000, Czech Republic

[3] E-Mails:

关键词: malignant melanoma;    angiogenesis;    nestin;    microvascular density;    CD90/Thy1;    FOXP3;   

DOI  :  10.3390/ijms16047876

来源: mdpi

【 摘 要 】

Malignant melanoma (MM) urgently needs identification of new markers with better predictive value than currently-used clinical and histological parameters. Cancer cells stimulate the formation of a specialized tumor microenvironment, which reciprocally affects uncontrolled proliferation and migration. However, this microenvironment is heterogeneous with different sub-compartments defined by their access to oxygen and nutrients. This study evaluated microvascular density (MVD), CD3⁺ lymphocytes (TILs) and FOXP3⁺ T-regulatory lymphocytes (Tregs) on formalin-fixed paraffin-embedded tissue sections using light microscopy. We analyzed 82 malignant melanomas, divided according to the AJCC TNM classification into four groups—pT1 (35), pT2 (17), pT3 (18) and pT4 (12)—and 25 benign pigmented nevi. All parameters were measured in both the central areas of tumors (C) and at their periphery (P). A marked increase in all parameters was found in melanomas compared to nevi (p = 0.0001). There was a positive correlation between MVD, TILs, FOXP3⁺ Tregs and the vertical growth phase. The results show that MVD, TILs and FOXP3⁺ Tregs substantially influence cutaneous melanoma microenvironment. We found significant topographic differences of the parameters between central areas of tumors and their boundaries.

【 授权许可】

CC BY   
© 2015 by the authors; licensee MDPI, Basel, Switzerland.