The effect of O-antigen length determinant wzz on the immunogenicity of Salmonella Typhimurium for Escherichia coli O2 O-polysaccharides delivery

Veterinary Research

Research Article

Pengju Chen¹  Anchun Cheng²  Changyong Cheng³  Jiali Xu³  Pu Wang³  Xindan Chen³  Simin Deng³  Qiyu Cao³  Jing Xia³  Ruidong Zhai³  Huan Zeng³  Yuji Chen³  Ping Luo³  Houhui Song³  Yue Han⁴ 

[1] Henan Institute of Morden Chinese Veterinary Medicine, 450002, Zhengzhou, China;Shangdong Xindehui Biotechnology Co., Ltd, 274700, Yunchengxian, China;Institute of Preventive Veterinary Medicine, College of Veterinary Medicine, Sichuan Agricultural University, 611130, Chengdu, China;Key Laboratory of Applied Technology On Green-Eco-Healthy Animal Husbandry of Zhejiang Province, Zhejiang Provincial Engineering Research Center for Animal Health Diagnostics & Advanced Technology, Zhejiang International Science and Technology Cooperation Base for Veterinary Medicine and Health Management, China-Australia Joint Laboratory for Animal, Health Big Data Analytics, College of Animal Science and Technology & College of Veterinary Medicine, Zhejiang A & F University, 666 Wusu Street, 311300, Hangzhou, China;Key Laboratory of Applied Technology On Green-Eco-Healthy Animal Husbandry of Zhejiang Province, Zhejiang Provincial Engineering Research Center for Animal Health Diagnostics & Advanced Technology, Zhejiang International Science and Technology Cooperation Base for Veterinary Medicine and Health Management, China-Australia Joint Laboratory for Animal, Health Big Data Analytics, College of Animal Science and Technology & College of Veterinary Medicine, Zhejiang A & F University, 666 Wusu Street, 311300, Hangzhou, China;Institute of Preventive Veterinary Medicine, College of Veterinary Medicine, Sichuan Agricultural University, 611130, Chengdu, China;

关键词: Salmonella;    lipopolysaccharide;    O-antigen chain length;    Escherichia coli;    immune response;   

DOI  :  10.1186/s13567-023-01142-4

 received in 2022-08-07, accepted in 2022-12-15,  发布年份 2022

来源: Springer

【 摘 要 】

Attenuated Salmonella Typhimurium is a promising antigen delivery system for live vaccines such as polysaccharides. The length of polysaccharides is a well-known key factor in modulating the immune response induced by glycoconjugates. However, the relationship between the length of Lipopolysaccharide (LPS) O-antigen (OAg) and the immunogenicity of S. Typhimurium remains unclear. In this study, we assessed the effect of OAg length determined by wzzST on Salmonella colonization, cell membrane permeability, antimicrobial activity, and immunogenicity by comparing the S. Typhimurium wild-type ATCC14028 strain to those with various OAg lengths of the ΔwzzST mutant and ΔwzzST::wzzECO2. The analysis of the OAg length distribution revealed that, except for the very long OAg, the short OAg length of 2–7 repeat units (RUs) was obtained from the ΔwzzST mutant, the intermediate OAg length of 13–21 RUs was gained from ΔwzzST::wzzECO2, and the long OAg length of over 20 RUs was gained from the wild-type. In addition, we found that the OAg length affected Salmonella colonization, cell permeability, and antibiotic resistance. Immunization of mice revealed that shortening the OAg length by altering wzzST had an effect on serum bactericidal ability, complement deposition, and humoral immune response. S. Typhimurium mutant strain ΔwzzST::wzzECO2 possessed good immunogenicity and was the optimum option for delivering E. coli O2 O-polysaccharides. Furthermore, the attenuated strain ATCC14028 ΔasdΔcrpΔcyaΔrfbPΔwzzST::wzzECO2-delivered E. coli O2 OAg gene cluster outperforms the ATCC14028 ΔasdΔcrpΔcyaΔrfbP in terms of IgG eliciting, cytokine expression, and immune protection in chickens. This study sheds light on the role of OAg length in Salmonella characteristics, which may have a potential application in optimizing the efficacy of delivered polysaccharide vaccines.

【 授权许可】

CC BY   
© The Author(s) 2023

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