Identification of histone acetyltransferase genes responsible for cannabinoid synthesis in hemp

Chinese Medicine

Research

Xiaotong Guo¹  Kang Ning²  Huatao Yu²  Linlin Dong²  Shilin Chen²  Cong Hou²  Yongzhong Chen²  Yufei Cheng³  Haibin Yu⁴ 

[1] College of Agronomy, Ludong University, 264000, Yantai, China;Key Laboratory of Beijing for Identification and Safety Evaluation of Chinese Medicine, Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, 100700, Beijing, China;Key Laboratory of Beijing for Identification and Safety Evaluation of Chinese Medicine, Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, 100700, Beijing, China;College of Agronomy, Ludong University, 264000, Yantai, China;Yunnan Hemp Industrial Investment CO.LTD, 650217, Kunming, China;

关键词: Histone acetyltransferase;    Hemp;    Acetylation;    Cannabinoid;    Abiotic stress;   

DOI  :  10.1186/s13020-023-00720-0

 received in 2022-10-23, accepted in 2023-01-31,  发布年份 2023

来源: Springer

【 摘 要 】

BackgroundHistone acetyltransferases (HATs) play an important role in plant growth and development, stress response, and regulation of secondary metabolite biosynthesis. Hemp (Cannabis sativa L.) is famous for its high industrial, nutritional, and medicinal value. It contains non-psychoactive cannabinoid cannabidiol (CBD) and cannabinol (CBG), which play important roles as anti-inflammatory and anti-anxiety. At present, the involvement of HATs in the regulation of cannabinoid CBD and CBG synthesis has not been clarified.MethodsThe members of HAT genes family in hemp were systematically analyzed by bioinformatics analysis. In addition, the expression level of HATs and the level of histone acetylation modification were analyzed based on transcriptome data and protein modification data. Real-time quantitative PCR was used to verify the changes in gene expression levels after inhibitor treatment. The changes of CBD and CBG contents after inhibitor treatment were verified by HPLC-MS analysis.ResultsHere, 11 HAT genes were identified in the hemp genome. Phylogenetic analysis showed that hemp HAT family genes can be divided into six groups. Cannabinoid synthesis genes exhibited spatiotemporal specificity, and histones were acetylated in different inflorescence developmental stages. The expression of cannabinoid synthesis genes was inhibited and the content of CBD and CBG declined by 10% to 55% in the samples treated by HAT inhibitor (PU139). Results indicated that CsHAT genes may regulate cannabinoid synthesis through altering histone acetylation.ConclusionsOur study provides genetic information of HATs responsible for cannabinoid synthesis, and offers a new approach for increasing the content of cannabinoid in hemp.

【 授权许可】

CC BY   
© The Author(s) 2023

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