Genome-wide identification and characterization of the lettuce GASA family in response to abiotic stresses

BMC Plant Biology

Research

Sun Ho Lee¹  Yong Weon Seo²  Dae Yeon Kim³  Woo Joo Jung⁴  Jin Seok Yoon⁵ 

[1] Department of Plant Biotechnology, Korea University, 02841, Seoul, Republic of Korea;Department of Plant Biotechnology, Korea University, 02841, Seoul, Republic of Korea;Ojeong Plant Breeding Research Center, Korea University, 02841, Seoul, Republic of Korea;Department of Plant Resources, College of Industrial Science, Kongju National University, 32439, Yesan, South Korea;Institute of Life Science and Natural Resources, Korea University, 02841, Seoul, Republic of Korea;Ojeong Plant Breeding Research Center, Korea University, 02841, Seoul, Republic of Korea;

关键词: Heat stress;    Lactuca sativa;    Shoot apical meristem;    cis;    Expression analysis;   

DOI  :  10.1186/s12870-023-04101-5

 received in 2022-12-01, accepted in 2023-02-01,  发布年份 2023

来源: Springer

【 摘 要 】

BackgroundLettuce is one of the most extensively farmed vegetables in the world, and it prefers cool growing conditions. High temperatures promote premature bolt formation, reducing quality and yield. The gibberellic acid-stimulated Arabidopsis (GASA) family genes play critical roles in plant growth, development, and stress responses. However, the biological functions of GASA proteins in lettuce have yet to be thoroughly investigated.ResultsUsing genome-wide analysis, 20 GASAs were identified in lettuce including, three groups of LsGASA proteins based on the phylogenetic analysis. Except for one, all GASA proteins included a conserved GASA domain with 12 cysteine residues. Cis-element analysis showed that LsGASAs were closely associated with light, phytohormones, and stress resistance. Five segmental and three tandem duplication events were observed in the LsGASA family based on duplication analysis. GASA synteny analysis among lettuce, Arabidopsis, tobacco, and rice revealed that LsGASA5 is highly collinear with all species. Six of the 20 LsGASA showed increased expression patterns at specific time points in the shoot apical meristem when subjected to heat stress. According to gene expression analysis, the majority of GASA were highly expressed in flowers compared to other organs, and six GASA exhibited highly increased expression levels in response to NaCl, abscisic acid, and gibberellin treatment. Furthermore, LsGASA proteins are predominantly found in the plasma membrane and/or the cytosol.ConclusionsThis study provides a comprehensive characterization of LsGASA genes for their diversity and biological functions. Moreover, our results will be useful for further studies on the function of lettuce GASA in abiotic stress- and heat-induced bolting signaling.

【 授权许可】

CC BY   
© The Author(s) 2023

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