Simple and rapid method for analysis of urinary vancomycin using solid phase extraction and fluorescence spectroscopy

AAPS Open

Research

Mizuki Hori¹  Miyu Matsumoto¹  Yuki Oshima²  Masaru Kato³ 

[1] Department of Pharmaceutical Sciences, School of Pharmacy, Showa University, 1-5-8 Hatanodai, Shinagawa-Ku, 142-8555, Tokyo, Japan;Devision of Bioanalytical Chemistry, Graduate School of Pharmacy, Showa University, 1-5-8 Hatanodai, Shinagawa-Ku, 142-8555, Tokyo, Japan;Devision of Bioanalytical Chemistry, Graduate School of Pharmacy, Showa University, 1-5-8 Hatanodai, Shinagawa-Ku, 142-8555, Tokyo, Japan;Department of Pharmaceutical Sciences, School of Pharmacy, Showa University, 1-5-8 Hatanodai, Shinagawa-Ku, 142-8555, Tokyo, Japan;

关键词: Fluorescence analysis;    Vancomycin;    Urine;    Dosing;    Therapeutic drug monitoring;   

DOI  :  10.1186/s41120-023-00071-y

 received in 2022-10-08, accepted in 2023-01-09,  发布年份 2023

来源: Springer

【 摘 要 】

Vancomycin (VCM) is an antimicrobial that is recommended for therapeutic drug monitoring (TDM) for maintaining the efficacy and safety of treatment. The trough monitoring has been used to guide VCM dosing regimens. However, newer guidelines recommend the use of area under the curve/minimum inhibitory concentration (AUC/MIC)-guided vancomycin dosing, and there is a need for easier and more frequent measurements of VCM concentrations. Therefore, in this study, we developed a simple and rapid analytical method for measuring urinary VCM by combining solid-phase extraction and fluorescence analysis. Urine samples are easier and less invasive than blood samples. In addition to the therapeutic range of blood VCM, this method was also able to measure 0.01–1 mg/mL, which is the concentration range of urinary VCM. The accuracy of 10, 20, and 30 μg/mL VCM solutions were between 93.18 and 109.76%. The relative standard deviation (RSD) of intra-day and inter-day analysis were less than 6.25% and 6.28%, respectively. Since this method does not use large equipment, it is expected to be better suited for clinical use.

【 授权许可】

CC BY   
© The Author(s) 2023

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