Protein Aggregation Measurement through Electrical Impedance Spectroscopy

2013 Joint IMEKO (International Measurement Confederation) TC1-TC7-TC13 Symposium: Measurement Across Physical and Behavioural Sciences

Affanni, A.^1 ; Corazza, A.^2 ; Esposito, G.^2 ; Fogolari, F.^2 ; Polano, M.^2

University of Udine, Department of Electrical, Management and Mechanical Engineering, via delle Scienze 206, 33100 Udine, Italy^1

University of Udine, Department of Medical and Biological Science, piazzale Kolbe 3, 33100 Udine, Italy^2

关键词: Aggregation process;    Electrical impedance spectroscopy;    Fibril formation;    Fluorescence analysis;    Impedance meters;    Inter-digitated electrodes;    Novel methodology;    Protein aggregation;   

Others  :  https://iopscience.iop.org/article/10.1088/1742-6596/459/1/012049/pdf
DOI  :  10.1088/1742-6596/459/1/012049

来源: IOP

【 摘 要 】

The paper presents a novel methodology to measure the fibril formation in protein solutions. We designed a bench consisting of a sensor having interdigitated electrodes, a PDMS hermetic reservoir and an impedance meter automatically driven by calculator. The impedance data are interpolated with a lumped elements model and their change over time can provide information on the aggregation process. Encouraging results have been obtained by testing the methodology on K-casein, a protein of milk, with and without the addition of a drug inhibiting the aggregation. The amount of sample needed to perform this measurement is by far lower than the amount needed by fluorescence analysis.