Biodegradable functionalized magnetite nanoparticles as binary-targeting carrier for breast carcinoma

BMC Chemistry

Research Article

Magda Ali Akl¹  Amira Mostafa Kamel²  Mahmoud Ahmed Abd El-Ghaffar² 

[1] Chemistry Department, Faculty of Science, Mansoura University, Mansoura, Egypt;Polymers and Pigments Department, National Research Centre, 33-El-Bohouth St. Dokki, Cairo, Egypt;

关键词: Superparamagnetic magnetite nanoparticles;    Doxorubicin hydrochloride;    Binary targeting drug system;    Breast carcinoma;   

DOI  :  10.1186/s13065-023-00915-4

 received in 2022-08-12, accepted in 2023-02-02,  发布年份 2023

来源: Springer

【 摘 要 】

In this study, Superparamagnetic magnetite nanoparticles (SPMNPs) are used in a new way as direct nanocarrier for Doxorubicin hydrochloride (DOX) via the functionalization of their surface with tri-sodium citrate through ligand exchange to conjugate DOX with imine bond to form tri-sodium citrate functionalized magnetite loaded DOX nanoparticles (DOX/Cit-MNPs). The DOX/Cit-MNPs were coated with chitosan to form chitosan coated citrate functionalized magnetite loaded DOX nanoparticles (Cs/DOX/Cit-MNPs) to offer biodegradability and pH-sensitive drug release features. The Fourier transform infrared spectroscopy (FTIR) analysis confirmed functionalization of SPMNPs, DOX-conjugation, and chitosan coating. The trans electron microscopy (TEM) show spherical nanostructures with average size 40 nm for coated nanocarriers. The saturation magnetization value of carrier was 59 emu/g.The in-vitro release of DOX from the chitosan coated tri-sodium citrate functionalized magnetite loaded DOX nanoparticles (Cs/DOX/Cit-MNPs) was studied to be 75% at pH 5.5 and 28.6% at pH 7.4 which proves the pH sensitivity of encapsulated Cs/DOX/Cit-MNPs. The effect of Cs/DOX/Cit-MNPs toward Human Breast Cancer Cell lines (MCF7) was studied and found to be 76% without magnet and 98% with external magnet after 72 h. With increasing DOX concentration and treatment time, the cell inhibition (IR%) of DOX solution and Cs/DOX-Cit-MNPs suspension to all cells is increased. Cs/DOX/Cit-MNPs showed sustained release and good inhibition to cancer cells and offer a protective mode for normal cells (WISH) compared to the free DOX.Graphical Abstract

【 授权许可】

CC BY   
© The Author(s) 2023

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