A multi-target protective effect of Danggui-Shaoyao-San on the vascular endothelium of atherosclerotic mice

BMC Complementary Medicine and Therapies

Research

Yuemeng Sun¹  Yang Tang¹  Yushan Gao¹  Yulin Zong¹  Fengjie Zheng¹  Yan Sun¹  Yixing Lu¹  Lu Zhou¹  Haoyu Zhu¹  Yuhang Li¹ 

[1] School of Chinese Medicine, Beijing University of Chinese Medicine, 100029, Beijing, China;

关键词: Danggui-Shaoyao-San;    Atherosclerosis;    Vascular endothelium;    Multiple targets;    Interα5β1/c-Abl/YAP;   

DOI  :  10.1186/s12906-023-03883-3

 received in 2022-11-15, accepted in 2023-02-11,  发布年份 2023

来源: Springer

【 摘 要 】

BackgroundAtherosclerosis (AS) is a chronic disease characterized by abnormal blood lipid metabolism, inflammation and vascular endothelial injury. Vascular endothelial injury is the initial stage during the occurrence of AS. However, the function and mechanism of anti-AS are not well characterized. Danggui-Shaoyao-San (DGSY) is a classic Traditional Chinese Medicine (TCM) prescription for the treatment of gynecological diseases, and has been widely used in the treatment of AS in recent years.MethodsApoE−/− atherosclerosis male mice were established by feeding with high-fat diet, and then randomly divided into three groups: Atherosclerosis group (AS), Danggui-Shaoyao-San group (DGSY), and Atorvastatin calcium group (X). The mice were administered with the drugs for 16 weeks. Pathological changes in aortic vessels were examined by staining with Oil red O, Masson and hematoxylin–eosin. In addition, blood lipids were analyzed. The level of IL-6 and IL-8 in aortic vessels were detected by ELISA and the expression of ICAM-1 and VCAM-1 in the aortic vascular endothelium were measured by Immunohistochemical. The mRNA expression of interα5β1/c-Abl/YAP in the aortic vessels were measured by Real-time quantitative PCR and location of expression was assessed by immunofluorescence.ResultsDGSY can significantly reduce the content of TC,TG and LDL-C and increase the level of HDL-C in the serum, reduce the plaque area and inhibit the concentration of IL-6 and IL-8, down-regulate the expression of IVAM-1,VCAM-1 and interα5β1/ c-Abl/YAP in the aortic vessels.ConclusionsCollectively, DGSY can alleviate vascular endothelium damage and delay the occurrence of AS, and the underlying mechanism may be related to the multi-target protective of DGSY.

【 授权许可】

CC BY   
© The Author(s) 2023

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