Encapsulation of Polygonum bistorta root phenolic compounds as a novel phytobiotic and its protective effects in the mouse model of enteropathogenic Escherichia coli infection

BMC Complementary Medicine and Therapies

Research

Negin Shafaei¹  Zahra kadkhoda Mezerji¹  Reza Boshrouyeh¹  Shaghayegh Ghajari¹  Ehsan Karimi¹  Hasti Hajiha¹  Seyedehfarnaz Hafezian Razavi¹  Ehsan Oskoueian² 

[1] Department of Biology, Mashhad Branch, Islamic Azad University, Mashhad, Iran;Department of Biology, Mashhad Branch, Islamic Azad University, Mashhad, Iran;Department of Research and Development, Arka Industrial Cluster, Mashhad, Iran;

关键词: Phytogenic;    Delivery;    Antibacterial agent;    Natural antibiotic;    Microencapsulation;    Antibiotic-alternative;   

DOI  :  10.1186/s12906-023-03868-2

 received in 2022-10-10, accepted in 2023-01-31,  发布年份 2023

来源: Springer

【 摘 要 】

BackgroundMicroencapsulation technology is the fundamental delivery system for encapsulating the natural bioactive compounds especially phenolic in order to developing bioavailability, stability and controlling release. This study was conducted to determine the antibacterial and health-promoting potential of the phenolic rich extract (PRE)-loaded microcapsules obtained from Polygonum bistorta root as a dietary phytobiotic in mice challenged by enteropathogenic Escherichia coli (E. coli).MethodThe PRE was obtained from Polygonum bistorta root using fractionation by different polarity solvents and the highest PRE was encapsulated by the combination of modified starch, maltodextrin, and whey protein concentrate as wall materials using a spray dryer. Then, the physicochemical characterization (particle size, zeta potential, Morphology and polydispersity index) of microcapsules have been assessed. For the invivo study, 30 mice at five treatment were designed and antibacterial properties were analyzed. Furthermore, relative fold changes in the ileum population of E. coli was investigated using Real time PCR.ResultsThe encapsulation of PRE resulted in the production of phenolic enriched extract-loaded microcapsules (PRE-LM) with a mean diameter of 330 nm and relatively high entrapment efficiency (87.2% w/v). The dietary supplementation of PRE-LM improved weight gain, liver enzymes, gene expression, morphometric characteristics of the ileum and decreased the population of E. coli present in the ileum significantly (p < 0.05).ConclusionOur funding suggested PRE-LM as a promising phytobiotic against E. coli infection in mice.

【 授权许可】

CC BY   
© The Author(s) 2023

【 图 表 】

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