Efficient production of cembratriene-ol in Escherichia coli via systematic optimization

Microbial Cell Factories

Research

Wei Zou¹  Kunjie Zhang²  Lei Chen²  Haiquan Yang²  Wei Shen³  Yuanyuan Xia³  Yu Cao³  Xianzhong Chen³ 

[1] College of Bioengineering, Sichuan University of Science & Engineering, 644000, Yibin, Sichuan, China;The Key Laboratory of Carbohydrate Chemistry and Biotechnology, School of Biotechnology, Ministry of Education, Jiangnan University, 214122, Wuxi, China;The Key Laboratory of Industrial Biotechnology, School of Biotechnology, Ministry of Education, Jiangnan University, 214122, Wuxi, China;

关键词: Escherichia coli;    DXP pathway;    Cembratriene-ol;    Key enzymes;    Systematicoptimization;   

DOI  :  10.1186/s12934-023-02022-4

 received in 2022-08-26, accepted in 2023-01-09,  发布年份 2023

来源: Springer

【 摘 要 】

BackgroundThe tobacco leaf–derived cembratriene-ol exhibits anti-insect effects, but its content in plants is scarce. Cembratriene-ol is difficult and inefficiently chemically synthesised due to its complex structure. Moreover, the titer of reported recombinant hosts producing cembratriene-ol was low and cannot be applied to industrial production.ResultsIn this study, Pantoea ananatis geranylgeranyl diphosphate synthase (CrtE) and Nicotiana tabacum cembratriene-ol synthase (CBTS) were heterologously expressed to synthsize the cembratriene-ol in Escherichia coli. Overexpression of cbts*, the 1-deoxy-d-xylulose 5-phosphate synthase gene dxs, and isopentenyl diphosphate isomerase gene idi promoted the production of cembratriene-ol. The cembratriene-ol titer was 1.53-folds higher than that of E. coli Z17 due to the systematic regulation of ggpps, cbts*, dxs, and idi expression. The production of cembratriene-ol was boosted via the overexpression of genes ispA, ispD, and ispF. The production level of cembratriene-ol in the optimal medium at 72 h was 8.55-folds higher than that before fermentation optimisation. The cembratriene-ol titer in the 15-L fermenter reached 371.2 mg L− 1, which was the highest titer reported.ConclusionIn this study, the production of cembratriene-ol in E. coli was significantly enhanced via systematic optimization. It was suggested that the recombinant E. coli producing cembratriene-ol constructed in this study has potential for industrial production and applications.

【 授权许可】

CC BY   
© The Author(s) 2023

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