Modulating and monitoring the functionality of corticostriatal circuits using an electrostimulable microfluidic device

Molecular Brain

Research

Nakwon Choi¹  Seokyoung Bang¹  Hong Nam Kim¹  Sukmin Han²  Sung Hyun Kim³ 

[1] Brain Science Institute, Korea Institute of Science and Technology (KIST), 02792, Seoul, Republic of Korea;Department of Neuroscience, Graduate School, Kyung Hee University, 02447, Seoul, Republic of Korea;Department of Neuroscience, Graduate School, Kyung Hee University, 02447, Seoul, Republic of Korea;Department of Physiology, School of Medicine, Kyung Hee University, 02447, Seoul, Republic of Korea;Medical Research Center for Bioreaction to Reactive Oxygen Species and Biomedical Science Institute, School of Medicine, Kyung Hee University, 02447, Seoul, South Korea;

关键词: Microfluidic device;    Corticostriatal (CStr) circuit;    Synapse;    Ca dynamics;    Action potential;    Synaptic transmission;   

DOI  :  10.1186/s13041-023-01007-z

 received in 2022-12-08, accepted in 2023-01-14,  发布年份 2023

来源: Springer

【 摘 要 】

The central nervous system is organized into different neural circuits, each with particular functions and properties. Studying neural circuits is essential to understanding brain function and neuronal diseases. Microfluidic systems are widely used for reconstructing and studying neural circuits but still need improvement to allow modulation and monitoring of the physiological properties of circuits. In this study, we constructed an improved microfluidic device that supports the electrical modulation of neural circuits and proper reassembly. We demonstrated that our microfluidic device provides a platform for electrically modulating and monitoring the physiological function of neural circuits with genetic indicators for synaptic functionality in corticostriatal (CStr) circuits. In particular, our microfluidic device measures activity-driven Ca2+ dynamics using Ca2+ indicators (synaptophysin-GCaMP6f and Fluo5F-AM), as well as activity-driven synaptic transmission and retrieval using vGlut-pHluorin. Overall, our findings indicate that the improved microfluidic platform described here is an invaluable tool for studying the physiological properties of specific neural circuits.

【 授权许可】

CC BY   
© The Author(s) 2023

【 图 表 】

41116_2022_35_Article_IEq339.gif

41116_2022_35_Article_IEq326.gif

41116_2022_35_Article_IEq319.gif

41116_2022_35_Article_IEq311.gif

41116_2022_35_Article_IEq304.gif

41116_2022_35_Article_IEq300.gif