Bulletin of the Korean chemical society | |
Microfluidic Image Cytometry (μFIC) Assessments of Silver Nanoparticle Cytotoxicity | |
Tae Hyun Yoon1  Jonghoon Park1  | |
关键词: Microfluidic image cytometry; Microfluidic device; Cytotoxicity assessment; Ag nanoparticles; Nanotoxicity; | |
DOI : | |
学科分类:化学(综合) | |
来源: Korean Chemical Society | |
【 摘 要 】
Cytotoxicity assessment of silver nanoparticles (AgNPs) was performed using MTT-based microfluidic image cytometry (μFIC). The LC50 value of HeLa cells exposed to AgNPs in the microfluidic device was estimated as 46.7 mg/L, which is similar to that estimated by MTT-based IC for cells cultured in a 96 well plate (49.9 mg/ L). These results confirm that the μFIC approach can produce cytotoxicity data that is reasonably well-matched with that of the conventional 96 well plate system with much higher efficiency. This μFIC method provides many benefits including ease of use and low cost, and is a more rapid in vitro cell based assay for AgNPs. This may aid in speeding up data acquisition in the field of nanosafety and make a significant contribution to the quantitative understanding of nanoproperty- toxicity relationships.
【 授权许可】
Unknown
【 预 览 】
Files | Size | Format | View |
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RO201912010243524ZK.pdf | 360KB | download |