Expression and function of myelin expression factor 2 in hepatocellular carcinoma

BMC Gastroenterology

Research

Peng Zhang¹  Jiang-Hua Zhao¹  Wei-Hua Cai²  Lin-Ling Ju³  Zhao-Lian Bian³  Hui-Xuan Wang³  Lin Chen³ 

[1]Medical School of Nantong University, Nantong Third People’s Hospital, 226000, Nantong, Jiangsu, People’s Republic of China

[2]Nantong Institute of Liver Disease, Department of Hepatobiliary Surgery, Nantong Third People’s Hospital, Nantong University, 226000, Nantong, Jiangsu, People’s Republic of China

[3]Nantong Institute of Liver Diseases, Nantong Third People’s Hospital, Nantong University, 226000, Nantong, Jiangsu, People’s Republic of China

关键词: Hepatocellular carcinoma;    MYEF2;    Prognosis;    Migration;    Invasion;   

DOI  :  10.1186/s12876-023-02644-3

 received in 2022-04-20, accepted in 2023-01-05,  发布年份 2023

来源: Springer

【 摘 要 】

IntroductionHepatocellular carcinoma (HCC) is one of the most common malignant tumours in the world and has a high mortality rate. However, the pathogenesis of HCC remains unclear. This study aimed to investigate the potential biomarkers of HCC.MethodsONCOMINE, HCCDB and THE HUMAN PROTEIN ATLAS were used to identify myelin expression factor 2 (MYEF2) as a potential biomarker for HCC. The Cancer Genome Atlas database was used to further validate and analyse the value of MYEF2. Kaplan–Meier Plotter was used for the prognostic analysis. The COX regression model and Kaplan–Meier method were used to investigate the clinical value of MYEF2 in the prognosis of HCC by reviewing the survival status of patients. Fluorescent quantitative polymerase chain reaction (qPCR) and immunohistochemistry were used to detect the expressions of the MYEF2 mRNA and protein in HCC tissues and cell lines. qPCR and Western blotting were used to validate the efficiency of MYEF2 knockout and overexpression in HCC cells. The invasion and migration abilities regulated by MYEF2 were detected by performing transwell and wound healing assays.ResultsMYEF2 is significantly upregulated in HCC and is mainly located in the nucleus of HCC cells. MYEF2 expression is significantly associated with the tumour stage, histological grade and TNM stage. High MYEF2 expression is an independent prognostic factor for patients with HCC. Functionally, elevated MYEF2 facilitated cell migration and invasion in vitro. In contrast, decreased MYEF2 inhibited cell migration and invasion.ConclusionsMYEF2 may be a novel biomarker with potential diagnosis and prognosis values and as a potential therapeutic target for HCC.

【 授权许可】

CC BY   
© The Author(s) 2023

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