Enhanced expression of miR-889 forecasts an unfavorable prognosis and facilitates cell progression in hepatocellular carcinoma

Diagnostic Pathology

He Wang¹  Huiwen Wang¹  Qi Zhang²  Jing Li²  Qiao Zhang²  Wenyu Cui² 

[1] Department of Interventional, Harbin Medical University Cancer Hospital, No.150 Haping Road, Harbin, 150081, Harbin, Heilongjiang, China;School of Pharmacy, Harbin University of Commerce, 138 Tongda Street, 150076, Heilongjiang, Harbin, China;

关键词: MiR-889;    Hepatocellular carcinoma;    Prognosis;    Proliferation;    Migration;    Invasion;   

DOI  :  10.1186/s13000-021-01111-5

来源: Springer

【 摘 要 】

BackgroundAs a new type of molecular marker, microRNAs (miRNAs) can be used for early diagnosis and prognosis prediction of malignant tumors, and has broad clinical application prospects. This paper mainly studies the important role of miR-889 in the occurrence and development of hepatocellular carcinoma and the prognostic significance of miR-889 in hepatocellular carcinoma.MethodsQuantitative real-time PCR analysis detected the expression levels of miR-889 in hepatocellular carcinoma tissues and cell lines. Kaplan-Meier curve and Cox regression analysis were used to explore the prognostic significance of miR-889 in hepatocellular carcinoma. The CCK-8 and Transwell assays assay were used to assess cell proliferation, migration, and invasion abilities ability.ResultsThe expression of miR-889 in hepatocellular carcinoma tissues was significantly higher than that in adjacent tissues. Overexpression of miR-889 was significantly associated with TNM stage, hepatitis B virus infection, and cirrhosis. Patients with high miR-889 expression had shorter overall survival than those with low miR-889 expression. And functional studies in two hepatocellular carcinoma cell lines have shown that overexpression of miR-889 significantly promoted cell proliferation, migration, and invasion in vitro.ConclusionsOverall, miR-889 was upregulated in hepatocellular carcinoma tissues and cell lines, and overexpression of miR-889 promoted cell proliferation, migration, and invasion in hepatocellular carcinoma cells. Based on our findings, high expression of miR-889 may promote the progression of hepatocellular carcinoma, and high expression of miR-889 is also forecasted for an unfavorable prognosis in hepatocellular carcinoma.

【 授权许可】

CC BY