Whole-transcriptome profiling across different developmental stages of Aedes albopictus (Diptera: Culicidae) provides insights into chitin-related non-coding RNA and competing endogenous RNA networks

Parasites & Vectors

Research

Wenjuan Liu¹  Kexin Zhang¹  Sha An¹  Zhong Zhang²  Ruiling Zhang²  Maoqing Gong³  Peng Cheng³ 

[1] School of Clinical and Basic Medical Sciences, Shandong First Medical University (Shandong Academy of Medical Sciences), 250117, Jinan, China;School of Clinical and Basic Medical Sciences, Shandong First Medical University (Shandong Academy of Medical Sciences), 250117, Jinan, China;School of Laboratory Animal (Shandong Laboratory Animal Center), Shandong First Medical University (Shandong Academy of Medical Sciences), 250117, Jinan, China;Shandong Institute of Parasitic Diseases, Shandong First Medical University (Shandong Academy of Medical Sciences), 272033, Jining, China;

关键词: ceRNA;    ncRNA;    Chitin;    Mosquito;   

DOI  :  10.1186/s13071-022-05648-2

 received in 2022-10-03, accepted in 2022-12-29,  发布年份 2022

来源: Springer

【 摘 要 】

BackgroundThe Asian tiger mosquito, Aedes albopictus, is one of the most invasive species and a vector of numerous arboviruses. The deleterious effects of long-term and inappropriate use of chemical pesticides have stimulated the exploration of new, environmentally friendly control strategies. Non-coding RNAs (ncRNAs) have been proven to participate in almost all biological processes of insects. MethodsIn this study, circular RNAs (circRNAs) and microRNAs (miRNAs) covering five developmental stages [egg, early larvae, late larvae, pupae, adult (female and male)] of A. albopictus were obtained using whole-transcriptome sequencing technology. Combined with long non-coding RNAs (lncRNAs) from previous research, circRNA/lncRNA‒miRNA‒mitochondrial RNA (mRNA) networks were constructed.ResultsA total of 1434 circRNAs and 208 miRNAs were identified. More differentially expressed circRNAs (DE circRNAs) and miRNAs (DE miRNAs) were found in the egg versus early larvae comparison group. Functional enrichment analysis demonstrated that most of the circRNA/lncRNA‒miRNA‒mRNA networks were involved in chitin metabolism. Hub genes of each circRNA/lncRNA‒miRNA‒mRNA network were screened out, which can be used as novel targets to disturb the molting process of A. albopictus.ConclusionsRegulatory relationships obtained from competing endogenous RNA (ceRNA) networks provide more information to manipulate the metamorphosis process and are helpful for developing effective and sustainable methods to control mosquitoes.Graphical Abstract

【 授权许可】

CC BY   
© The Author(s) 2023

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