The potential activity of biosynthesized silver nanoparticles of Pseudomonas aeruginosa as an antibacterial agent against multidrug-resistant isolates from intensive care unit and anticancer agent

Environmental Sciences Europe

Research

Magdy Kamal Mohammed Khalil¹  Rateb Nabil Abbas²  A. B. Abeer Mohammed²  Abdallah Soubhy Ali³  Mona Mohamed Abd Elhamid⁴ 

[1] Anesthesia and Intensive Care Department, National Liver Institute, Menoufia University, 32511, Shebin El-Kom, Egypt;Department of Microbial Biotechnology, Genetic Engineering and Biotechnology Research Institute, University of Sadat City, Sadat, Egypt;Department of Microbiology, Faculty of Agriculture, Cairo University, 12613, Giza, Egypt;National Liver Institute, Menoufia University, 32511, Shebin El-Kom, Egypt;

关键词: Silver nanoparticles;    Multidrug-resistant bacteria;    Pseudomonas aeruginosa;    Cytotoxicity;    Hepatocellular carcinoma;   

DOI  :  10.1186/s12302-022-00684-2

 received in 2022-05-25, accepted in 2022-10-02,  发布年份 2022

来源: Springer

【 摘 要 】

BackgroundAntibiotic resistance is a global problem; especially the multidrug-resistant bacteria are a serious and fatal problem in the intensive care unit. Interestingly, biosynthesized silver nanoparticles are the promising key to eliminate these microbes. Using Pseudomonas aeruginosa supernatant is an easy and cheap method in silver nanoparticle biosynthesis. The biosynthesis conditions were adjusted, and the profiling of the biosynthesized silver nanoparticles was confirmed.ResultsThe UV spectroscopy at a wavelength at 400 nm was 0.539 A.U., transmission electron microscope showed nanoparticles were homogeneous with a square and spherical shape, its average size 20 nm, The capping material and the existence of silver nanoparticles were confirmed using Fourier transform infrared spectroscopy, and energy dispersive X-ray spectroscopy. The minimum inhibitory concentration was 1 mg/ml against multidrug-resistant bacteria, and LC50 was 62.307 μg/ml on the hepatocellular carcinoma cell line.ConclusionsMicrobial-synthesized silver nanoparticles have a potential application to combat multidrug-resistant bacteria, especially in the intensive care unit.

【 授权许可】

CC BY   
© The Author(s) 2022. corrected publication 2023

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