Evaluation the efficacy of oral immunization of broiler chickens with a recombinant Lactobacillus casei vaccine vector expressing the Carboxy-terminal fragment of α-toxin from Clostridium perfringens

BMC Veterinary Research

Research

Mehdi Golchin¹  Mohammad Ali Shamshirgaran¹  Reza Kheirandish²  Mahmoud Salehi³ 

[1] Division of Microbiology, Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, Post Box: 76169-133, 7616914111, Kerman, Iran;Division of Pathology, Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran;Division of Poultry Diseases, Department of Clinical Science, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran;

关键词: α-Toxin;    Clostridium perfringens;    Lactobacillus casei;    Necrotic enteritis;    Probiotic;    Vector vaccine;   

DOI  :  10.1186/s12917-023-03566-8

 received in 2022-10-08, accepted in 2023-01-04,  发布年份 2023

来源: Springer

【 摘 要 】

BackgroundClostridium perfringens (C. perfringens) is a serious anaerobic enteric pathogen causing necrotic enteritis (NE) in broiler chickens. Following the ban on antibiotics as growth promoters in animal feedstuffs, there has been a remarkable rise in occurrence of NE which resulted in considering alternative approaches, particularly vaccination. The objective of this work was to evaluate the recombinant Lactobacillus casei (L. casei) expressing the C-terminal domain of α-toxin from C. perfringens as a potential probiotic-based vaccine candidate to immunize the broiler chickens against NE.ResultsThe broiler chickens immunized orally with recombinant vaccine strain were significantly protected against experimental NE challenge, and developed specific serum anti-α antibodies. Additionally, the immunized birds showed higher body weight gains compared with control groups during the challenge experiment.ConclusionsThe current study showed that oral immunization of broiler chickens with a safe probiotic-based vector vaccine expressing α-toxin from C. perfringens could provide protective immunity against NE in birds.

【 授权许可】

CC BY   
© The Author(s) 2023

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