Study of microencapsulated fatty acid antimicrobial activity in vitro and its prevention ability of Clostridium perfringens induced necrotic enteritis in broiler chicken

Gut Pathogens

Research

Youfang Gu¹  Shilin Wu²  Fangquan Yan²  Bing Chen²  Minna Lv³  Haiming Cai³  Junjing Hu³  Juan Li³  Jianfei Zhang³  Nanshan Qi³  Mingfei Sun³  Xuhui Lin³  Shenquan Liao³  Shaobing Liu⁴  Zhuanqiang Yan⁵  Qingfeng Zhou⁵ 

[1] College of Animal Science and Technology, Anhui Science and Technology University, 233100, Fengyang, Anhui, People’s Republic of China;Guangzhou Wisdom Bio-Technology Co., Ltd, 510700, Guangzhou, Guangdong, People’s Republic of China;Key Laboratory of Livestock Disease Prevention of Guangdong Province, Key Laboratory of Avian Influenza and Other Major Poultry Diseases Prevention and Control, Ministry of Agriculture and Rural Affairs, Institute of Animal Health, Guangdong Academy of Agricultural Sciences, 510640, Guangzhou, Guangdong, People’s Republic of China;Key Laboratory of Livestock Disease Prevention of Guangdong Province, Key Laboratory of Avian Influenza and Other Major Poultry Diseases Prevention and Control, Ministry of Agriculture and Rural Affairs, Institute of Animal Health, Guangdong Academy of Agricultural Sciences, 510640, Guangzhou, Guangdong, People’s Republic of China;College of Animal Science and Technology, Anhui Science and Technology University, 233100, Fengyang, Anhui, People’s Republic of China;Wen’s Group Academy, Wen’s Foodstuffs Group Co., Ltd., 527400, Xinxing, Guangdong, People’s Republic of China;

关键词: Necrotic enteritis;    Clostridium perfringens;    Medium chain fatty acid;    Antimicrobial activity;    Broiler chicken;   

DOI  :  10.1186/s13099-022-00526-9

 received in 2022-11-19, accepted in 2022-12-19,  发布年份 2022

来源: Springer

【 摘 要 】

BackgroundNecrotic enteritis (NE) is an infectious intestinal disease caused by Clostridium perfringens (C. perfringens) that is now re-emerging and causing concern within the poultry industry. Previously, the supplementation of antibiotics in feed was the most popular control strategy against C. perfringens. However, with the ban on supplementing growth-promoting antibiotics in livestock feed, alternatives to antibiotics will be essential in order to control necrotic enteritis. A possible alternative to antibiotics could be the medium or long chain fatty acids (MCFA or LCFA) as these are able to destroy cell membranes which in turn results in the death of bacteria. In this study, the in vitro antimicrobial activity of different combinations with microencapsulated caprylic acid (C8: 0), capric acid (C10: 0), lauric acid (C12: 0) and myristic acid (C14: 0) against C. perfringens and in vivo control the NE-inducing C. perfringens in broiler chicken were analyzed.ResultsThe minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC) assay results revealed that three different combinations of medium/long chain fatty acids varied in antimicrobial activities against C. perfringens type A strain (CVCC52, quality control), C. perfringens type A strain (C8-1), C. perfringens type G strain (D25) and C. perfringens type G strain (MZ1). Specifically, combination of C12: 0 and C14: 0 (C12–14) showed the highest antimicrobial activity against the four strains of C. perfringens (MIC ≤ 12.5 μg/mL, MBC = 50 μg/mL), followed by the combination of C10: 0 and C12: 0 (C10–12) (MIC, MBC ≤ 50 μg/mL). The in vivo study, 189 of 818-crossbred chickens that were fed a wheat-based diet and randomly divided into nine groups, with six treatment groups supplemented with either a high dose (1 g/kg) or low dose (0.5 g/kg) of three combinations respectively. The remaining three groups comsisted of a positive group supplement with avilamycin (0.01 g/kg), an infected control and an uninfected control. All chickens were challenged with C. perfringens from day 14 to day 17, except those in the uninfected control group. On day 20, the duodenum and jejunum necrotic lesions scores were calculated and the results showed that there was significant decrease in the C12–C14 high dose group (1.43 ± 0.23, 0.48 ± 0.13) and the C10–12 high dose group (1.52 ± 0.19, 0.48 ± 0.11) compared to the infected group (2.86 ± 0.21, 1.20 ± 0.28).ConclusionsThis finding indicated that dietary microencapsulated C12–C14 and C10–C12 could inhibit the growth of C. perfringens in chickens, which proves is viability to serve as an alternative to antibiotics used for necrotic enteritis caused by C. perfringens.

【 授权许可】

CC BY   
© The Author(s) 2023

【 图 表 】

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