Genome wide identification of GDSL gene family explores a novel GhirGDSL26 gene enhancing drought stress tolerance in cotton

BMC Plant Biology

Research

Aziz Khan¹  Jiajun Liu²  Jiangna Liu²  Yuqing Hou²  Zhongli Zhou²  Yanchao Xu²  Yuhong Wang²  Heng Wang²  Jie Zheng³  Xiaoyan Cai⁴  Fang Liu⁵ 

[1] Key Laboratory of Plant Genetics and Breeding, College of Agriculture, Guangxi University, 530005, Nanning, China;State Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, 455000, Anyang, China;State Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, 455000, Anyang, China;Hainan Yazhou Bay Seed Laboratory, 572024, Sanya, China;National Nanfan Research Institute (Sanya), Chinese Academy of Agriculture Sciences, 572025, Sanya, China;State Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, 455000, Anyang, China;National Nanfan Research Institute (Sanya), Chinese Academy of Agriculture Sciences, 572025, Sanya, China;State Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, 455000, Anyang, China;School of Agricultural Sciences, Zhengzhou University, Zhengzhou, China;

关键词: Cotton;    GDSL;    Drought resistance;    Functional identification;   

DOI  :  10.1186/s12870-022-04001-0

 received in 2022-10-10, accepted in 2022-12-13,  发布年份 2022

来源: Springer

【 摘 要 】

BackgroundCurrent climate change scenarios are posing greater threats to the growth and development of plants. Thus, significant efforts are required that can mitigate the negative effects of drought on the cotton plant. GDSL esterase/lipases can offer an imperative role in plant development and stress tolerance. However, thesystematic and functional roles of the GDSL gene family, particularly in cotton under water deficit conditions have not yet been explored.ResultsIn this study, 103, 103, 99, 198, 203, 239, 249, and 215 GDSL proteins were identified in eight cotton genomes i.e., Gossypium herbaceum (A1), Gossypium arboretum (A2), Gossypium raimondii (D5), Gossypium hirsutum (AD1), Gossypium barbadense (AD2), Gossypium tomentosum (AD3), Gossypium mustelinum (AD4), Gossypium darwinii (AD5), respectively. A total of 198 GDSL genes of Gossypium hirsutum were divided into eleven clades using phylogenetic analysis, and the number of GhirGDSL varied among different clades. The cis-elements analysis showed that GhirGDSL gene expression was mainly related to light, plant hormones, and variable tense environments. Combining the results of transcriptome and RT-qPCR, GhirGDSL26 (Gh_A01G1774), a highly up-regulated gene, was selected for further elucidating its tole in drought stress tolerance via estimating physiological and biochemical parameters. Heterologous expression of the GhirGDSL26 gene in Arabidopsis thaliana resulted in a higher germination and survival rates, longer root lengths, lower ion leakage and induced stress-responsive genes expression under drought stress. This further highlighted that overexpressed plants had a better drought tolerance as compared to the wildtype plants. Moreover, 3, 3’-diaminobenzidine (DAB) and Trypan staining results indicated reduced oxidative damage, less cell membrane damage, and lower ion leakage in overexpressed plants as compared to wild type. Silencing of GhirGDSL26 in cotton via VIGS resulting in a susceptible phenotype, higher MDA and H2O2 contents, lower SOD activity, and proline content.ConclusionOur results demonstrated that GhirGDSL26 plays a critical role in cotton drought stress tolerance. Current findings enrich our knowledge of GDSL genes in cotton and provide theoretical guidance and excellent gene resources for improving drought tolerance in cotton.

【 授权许可】

CC BY   
© The Author(s) 2023

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