Single-cell RNA sequencing revealed the liver heterogeneity between egg-laying duck and ceased-laying duck

BMC Genomics

Research

Xiaoqin Xu¹  Wanqiu Zhao²  Shujing Lai³  Yong Tian⁴  Tao Zeng⁴  Wenwu Xu⁴  Lizhi Lu⁴  Xue Du⁵ 

[1] Institute of Ecology, China West Normal University, 637002, Nanchong, Sichuan, China;Institute of Horticulture, Zhejiang Academy of Agricultural Sciences, 310022, Hangzhou, Zhejiang, China;Shanghai Institute of Immunology, Shanghai Jiao Tong University School of Medicine, Shanghai, China;State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Institute of Animal Husbandry and Veterinary Medicine, Zhejiang Academy of Agricultural Sciences, 310021, Hangzhou, Zhejiang, China;State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Institute of Animal Husbandry and Veterinary Medicine, Zhejiang Academy of Agricultural Sciences, 310021, Hangzhou, Zhejiang, China;College of Animal Science and Technology, College of Veterinary Medicine, Zhejiang A & F University, Hangzhou, China;

关键词: Ceased-laying duck;    Single-cell RNA-seq;    Cell heterogeneity;    Hepatocytes;    Cell cluster;   

DOI  :  10.1186/s12864-022-09089-0

 received in 2022-09-19, accepted in 2022-12-19,  发布年份 2022

来源: Springer

【 摘 要 】

BackgroundIn the late phase of production, ducks untimely cease laying, leading to a lower feed conversion. Liver plays a vital role in the synthesis and transport of yolk materials during egg formation in birds. However, the molecular mechanism of liver in ceased-laying duck is far from clear, higher resolution and deeper analysis is needed. Sing-cell RNA-sequencing of 10 × Genomics platform can help to map the liver single cell gene expression atlas of Shaoxing duck and provide new insights into the liver between egg-laying and ceased-laying ducks.ResultsAbout 20,000 single cells were profiled and 22 clusters were identified. All the clusters were identified as 6 cell types. The dominant cell type is hepatocyte, accounted for about 60% of all the cells. Of note, the heterogeneity of cells between egg-laying duck and ceased-laying duck mainly occurred in hepatocytes. Cells of cluster 3 and 12 were the unique hepatocyte states of egg-laying ducks, while cells of cluster 0 and 15 were the unique hepatocyte states of ceased-laying ducks. The expression mode of yolk precursor transporters, lipid metabolizing enzymes and fibrinogens were different in hepatocytes between egg-laying duck and ceased-laying duck. APOV1, VTG2, VTG1, APOB, RBP, VTDB and SCD might be activated in egg-laying ducks, while APOA1, APOA4, APOC3, FGB and FGG might be activated in ceased-laying ducks.ConclusionsOur study further proofs that APOV1 and APOB play key roles in egg production, rather than APOA1 and APOA4. It is also the first to detect a correlation between the higher expression of APOC3, FGB, FGG and ceased-laying in duck.

【 授权许可】

CC BY   
© The Author(s) 2022

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