LncRNA HOTAIR impairs the prognosis of papillary thyroid cancer via regulating cellular malignancy and epigenetically suppressing DLX1

Cancer Cell International

Research

Yu-Ting Wang¹  Yao-Feng Li²  Chien-Ming Lin³  Sheng-Chiang Su⁴  Li-Ju Ho⁴  Feng-Chih Kuo⁴  Chia-Luen Huang⁴  Jhih-Syuan Liu⁴  Chieh-Hua Lu⁴  Peng-Fei Li⁴  Chien-Hsing Lee⁵  Chia-Hsin Liu⁶ 

[1] Department and Graduate Institute of Life Biochemistry, National Defense Medical Center, Taipei, Taiwan;Department of Pathology, Tri-Service General Hospital, National Defense Medical Center, Taipei, Taiwan;Department of Pediatrics, Tri-Service General Hospital, National Defense Medical Center, Taipei, Taiwan;Division of Endocrinology and Metabolism, Department of Internal Medicine, Tri-Service General Hospital, National Defense Medical Center, Taipei, Taiwan;Division of Endocrinology and Metabolism, Department of Internal Medicine, Tri-Service General Hospital, National Defense Medical Center, Taipei, Taiwan;Department and Graduate Institute of Life Biochemistry, National Defense Medical Center, Taipei, Taiwan;Division of Pulmonary and Critical Care Medicine, Department of Internal Medicine, Tri-Service General Hospital, National Defense Medical Center, Taipei, Taiwan;

关键词: lncRNA;    HOTAIR;    Papillary thyroid cancer;    DLX1;   

DOI  :  10.1186/s12935-022-02817-2

 received in 2022-05-12, accepted in 2022-11-30,  发布年份 2022

来源: Springer

【 摘 要 】

PurposePapillary thyroid cancer (PTC) is the most common endocrine malignancy with a fast-growing incidence in recent decades. HOTAIR as a long non-coding RNA has been shown to be highly expressed in papillary thyroid cancer tissues with only a limited understanding of its functional roles and downstream regulatory mechanisms in papillary thyroid cancer cells.MethodsWe applied three thyroid cancer cell lines (MDA-T32, MDA-T41 and K1) to investigate the phenotypic influence after gain or loss of HOTAIR. The Cancer Genome Atlas (TCGA) database were utilised to select candidate genes possibly regulated by HOTAIR with validation in the cellular system and immunohistochemical (IHC) staining of PTC tissues.ResultsWe observed HOTAIR was highly expressed in MDA-T32 cells but presents significantly decreased levels in MDA-T41 and K1 cells. HOTAIR knockdown in MDA-T32 cells significantly suppressed proliferation, colony formation, migration with cell cycle retardation at G1 phase. On the contrary, HOTAIR overexpression in MDA-T41 cells dramatically enhanced proliferation, colony formation, migration with cell cycle driven toward S and G2/M phases. Similar phenotypic effects were also observed as overexpressing HOTAIR in K1 cells. To explore novel HOTAIR downstream mechanisms, we analyzed TCGA transcriptome in PTC tissues and found DLX1 negatively correlated to HOTAIR, and its lower expression associated with reduced progression free survival. We further validated DLX1 gene was epigenetically suppressed by HOTAIR via performing chromatin immunoprecipitation. Moreover, IHC staining shows a significantly stepwise decrease of DLX1 protein from normal thyroid tissues to stage III PTC tissues.ConclusionsOur study pointed out that HOTAIR is a key regulator of cellular malignancy and its epigenetic suppression on DLX1 serves as a novel biomarker to evaluate the PTC disease progression.

【 授权许可】

CC BY   
© The Author(s) 2022

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