Identification of the miRNA-mRNA regulatory network associated with radiosensitivity in esophageal cancer based on integrative analysis of the TCGA and GEO data

BMC Medical Genomics

Research

Li Ren¹  Qing Qin¹  Hongmin Chen¹  Yuming Wan¹  Li Zeng¹  Hongyu Zhuo¹  Lin Zhou²  Xiaoxiao Shi³  Wangmu Sangdan⁴ 

[1] Cancer Center, West China Hospital of Sichuan University, No. 37 Guoxue Lane, Wuhou District, 610041, Chengdu, People’s Republic of China;Cancer Center, West China Hospital of Sichuan University, No. 37 Guoxue Lane, Wuhou District, 610041, Chengdu, People’s Republic of China;Department of Thoracic Oncology, State Key Laboratory of Biotherapy, Sichuan University, No. 1, Keyuan 4th Road, Gaopeng Avenue, 610041, Chengdu, People’s Republic of China;Department of Medical Oncology, Chengdu Shang Jin Nan Fu Hospital (West China Hospital, S.C.U.), 611730, Chengdu, People’s Republic of China;Department of Oncology, People’s Hospital of Tibet Autonomous Region, 850000, Lhasa, People’s Republic of China;

关键词: Esophageal cancer;    Radiosensitivity;    miRNA-mRNA regulatory network;    miR-132-3p;    CAND1;    ZDHHC23;    miR-576-5p;    AHR;   

DOI  :  10.1186/s12920-022-01392-9

 received in 2022-05-23, accepted in 2022-11-07,  发布年份 2022

来源: Springer

【 摘 要 】

BackgroundThe current study set out to identify the miRNA-mRNA regulatory networks that influence the radiosensitivity in esophageal cancer based on the The Cancer Genome Atlas (TCGA) and the Gene Expression Omnibus (GEO) databases.MethodsFirstly, esophageal cancer-related miRNA-seq and mRNA-seq data were retrieved from the TCGA database, and the mRNA dataset of esophageal cancer radiotherapy was downloaded from the GEO database to analyze the differential expressed miRNAs (DEmiRNAs) and mRNAs (DEmRNAs) in radiosensitive and radioresistant samples, followed by the construction of the miRNA-mRNA regulatory network and Gene Ontology and KEGG enrichment analysis. Additionally, a prognostic risk model was constructed, and its accuracy was evaluated by means of receiver operating characteristic analysis.ResultsA total of 125 DEmiRNAs and 42 DEmRNAs were closely related to the radiosensitivity in patients with esophageal cancer. Based on 47 miRNA-mRNA interactions, including 21 miRNAs and 21 mRNAs, the miRNA-mRNA regulatory network was constructed. The prognostic risk model based on 2 miRNAs (miR-132-3p and miR-576-5p) and 4 mRNAs (CAND1, ZDHHC23, AHR, and MTMR4) could accurately predict the prognosis of esophageal cancer patients. Finally, it was verified that miR-132-3p/CAND1/ZDHHC23 and miR-576-5p/AHR could affect the radiosensitivity in esophageal cancer.ConclusionOur study demonstrated that miR-132-3p/CAND1/ZDHHC23 and miR-576-5p/AHR were critical molecular pathways related to the radiosensitivity of esophageal cancer.

【 授权许可】

CC BY   
© The Author(s) 2022

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