Is Increased Low-dose somatic Radiosensitivity Associated with Increased Transgenerational Germline Mutation

Brenner, David J.

关键词: BONE MARROW;    DNA;    MALES;    MICE;    MUTATION FREQUENCY;    MUTATIONS;    POLYMERASE CHAIN REACTION;    RADIOSENSITIVITY;    SPERMATOZOA;    SPONTANEOUS MUTATIONS Low-Dose;    Radiosensitivity;   

DOI  :  10.2172/938982
RP-ID  :  DOE/ER/63631
PID  :  OSTI ID: 938982
Others  :  TRN: US0902919

学科分类：核能源与工程

美国|英语

来源: SciTech Connect

【 摘 要 】

Using single-molecule polymerase chain reaction, the frequency of spontaneous and radiation-induced mutation at an expanded simple tandem repeat (ESTR) locus was studied in DNA samples extracted from sperm and bone marrow of Atm knockout (Atm+/–) heterozygous male mice. The frequency of spontaneous mutation in sperm and bone marrow in Atm+/– males did not significantly differ from that in wild-type BALB/c mice. Acute gamma-ray exposure did not affect ESTR mutation frequency in bone marrow and resulted in similar increases in sperm samples taken from Atm+/– and BALB/c males. Taken together, these results suggest that the Atm haploinsufficiency analyzed in our study does not affect spontaneous and radiation-induced ESTR mutation frequency in mice.